Cloned (Comment) | Organism |
---|---|
gene murI from strain NC-8, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)/pET20b-murI | Lactiplantibacillus plantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.84 | - |
L-glutamate | pH 7.5, 37°C | Lactiplantibacillus plantarum | |
1.64 | - |
D-glutamate | pH 7.5, 37°C | Lactiplantibacillus plantarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, recombinant enzyme, SDS-PAGE | Lactiplantibacillus plantarum |
55800 | - |
recombinant enzyme, gel filtration | Lactiplantibacillus plantarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Lactiplantibacillus plantarum | - |
D-glutamate | - |
r | |
L-glutamate | Lactiplantibacillus plantarum NC8 | - |
D-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | D7VBA0 | gene murI | - |
Lactiplantibacillus plantarum NC8 | D7VBA0 | gene murI | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 9.16fold from Escherichia coli strain BL21(DE3)/pET20b-murI by L-Glu affinity and anion exchange chromatography | Lactiplantibacillus plantarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2043 | - |
purified recombinant enzyme, pH 7.5, 37°C | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glutamate | - |
Lactiplantibacillus plantarum | L-glutamate | - |
r | |
D-glutamate | - |
Lactiplantibacillus plantarum NC8 | L-glutamate | - |
r | |
L-glutamate | - |
Lactiplantibacillus plantarum | D-glutamate | - |
r | |
L-glutamate | - |
Lactiplantibacillus plantarum NC8 | D-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, recombinant enzyme, SDS-PAGE | Lactiplantibacillus plantarum |
Synonyms | Comment | Organism |
---|---|---|
glutamic acid racemases | - |
Lactiplantibacillus plantarum |
MurI | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Lactiplantibacillus plantarum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | activity range, optimum at 50°C, inactive at 55°C | Lactiplantibacillus plantarum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3029 | - |
L-glutamate | pH 7.5, 37°C | Lactiplantibacillus plantarum | |
5980 | - |
D-glutamate | pH 7.5, 37°C | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Lactiplantibacillus plantarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | activity range | Lactiplantibacillus plantarum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | 97% activity remaining after 22 h at pH 7.0-8.0, half-life, by extrapolation of the data, is 287 h at pH 7.0, 154 h at pH 8.0, 25 h at pH 9.0, and 6 h at pH 10.0 | Lactiplantibacillus plantarum |
General Information | Comment | Organism |
---|---|---|
additional information | MurI from Lactobacillus plantarum NC8 seems to exhibit pseudosymmetry for the racemisation of Glu in both directions | Lactiplantibacillus plantarum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3606 | - |
L-glutamate | pH 7.5, 37°C | Lactiplantibacillus plantarum | |
3646 | - |
D-glutamate | pH 7.5, 37°C | Lactiplantibacillus plantarum |