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Literature summary for 5.1.1.3 extracted from

  • Boehmer, N.; Dautel, A.; Eisele, T.; Fischer, L.
    Recombinant expression, purification and characterisation of the native glutamate racemase from Lactobacillus plantarum NC8 (2013), Protein Expr. Purif., 88, 54-60.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene murI from strain NC-8, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)/pET20b-murI Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.84
-
L-glutamate pH 7.5, 37°C Lactiplantibacillus plantarum
1.64
-
D-glutamate pH 7.5, 37°C Lactiplantibacillus plantarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
2 * 30000, recombinant enzyme, SDS-PAGE Lactiplantibacillus plantarum
55800
-
recombinant enzyme, gel filtration Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Lactiplantibacillus plantarum
-
D-glutamate
-
r
L-glutamate Lactiplantibacillus plantarum NC8
-
D-glutamate
-
r

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum D7VBA0 gene murI
-
Lactiplantibacillus plantarum NC8 D7VBA0 gene murI
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 9.16fold from Escherichia coli strain BL21(DE3)/pET20b-murI by L-Glu affinity and anion exchange chromatography Lactiplantibacillus plantarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2043
-
purified recombinant enzyme, pH 7.5, 37°C Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glutamate
-
Lactiplantibacillus plantarum L-glutamate
-
r
D-glutamate
-
Lactiplantibacillus plantarum NC8 L-glutamate
-
r
L-glutamate
-
Lactiplantibacillus plantarum D-glutamate
-
r
L-glutamate
-
Lactiplantibacillus plantarum NC8 D-glutamate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 30000, recombinant enzyme, SDS-PAGE Lactiplantibacillus plantarum

Synonyms

Synonyms Comment Organism
glutamic acid racemases
-
Lactiplantibacillus plantarum
MurI
-
Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Lactiplantibacillus plantarum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 activity range, optimum at 50°C, inactive at 55°C Lactiplantibacillus plantarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3029
-
L-glutamate pH 7.5, 37°C Lactiplantibacillus plantarum
5980
-
D-glutamate pH 7.5, 37°C Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Lactiplantibacillus plantarum

pH Range

pH Minimum pH Maximum Comment Organism
7 10 activity range Lactiplantibacillus plantarum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 97% activity remaining after 22 h at pH 7.0-8.0, half-life, by extrapolation of the data, is 287 h at pH 7.0, 154 h at pH 8.0, 25 h at pH 9.0, and 6 h at pH 10.0 Lactiplantibacillus plantarum

General Information

General Information Comment Organism
additional information MurI from Lactobacillus plantarum NC8 seems to exhibit pseudosymmetry for the racemisation of Glu in both directions Lactiplantibacillus plantarum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3606
-
L-glutamate pH 7.5, 37°C Lactiplantibacillus plantarum
3646
-
D-glutamate pH 7.5, 37°C Lactiplantibacillus plantarum