Cloned (Comment) | Organism |
---|---|
cloned in Escherichia coli as a fusion protein with a 6x His tag at the N-terminus | Streptococcus pyogenes |
Crystallization (Comment) | Organism |
---|---|
crystallization is carried out by the sitting-drop,vapor-diffusion method, crystal structures of GluR from Streptococcus pyogenes in both inhibitor-free and inhibitor-bound forms. The inhibitor-free GluR crystallizes in two different forms, which diffracts to 2.25 A and 2.5 A resolution, while the inhibitor-bound crystal diffracted to 2.5 A resolution. | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
gamma-2-naphthylmethyl-D-glutamate | potent competitive inhibitor that induces a disorder in one of the loops near the active site | Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.74 | - |
L-glutamate | - |
Streptococcus pyogenes | |
126 | - |
D-glutamate | - |
Streptococcus pyogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
method not indicated | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | Q9A1B7 | - |
- |
Purification (Comment) | Organism |
---|---|
protein extract is purified by using a nickel-chelated Hi-trap chelating column, Hi-trap sp-FF cation-exchange column and by by gel-filtration on a Superdex-200 prep-grade column | Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Streptococcus pyogenes | D-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | crystal structure analysis, GluR is composed of two domains of alpha/beta protein that are related by pseudo-2fold symmetry and the active site is located at the domain interface | Streptococcus pyogenes |
Synonyms | Comment | Organism |
---|---|---|
GluR | - |
Streptococcus pyogenes |
glutamate racemase | - |
Streptococcus pyogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptococcus pyogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
D-glutamate | - |
Streptococcus pyogenes | |
1.63 | - |
L-glutamate | - |
Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptococcus pyogenes |