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Literature summary for 5.1.1.3 extracted from

  • Oikawa, T.; Watanabe, M.; Makiura, H.; Kusakabe, H.; Yamade, K.; Soda, K.
    Production of D-glutamate from L-glutamate with glutamate racemase and L-glutamate oxidase (1999), Biosci. Biotechnol. Biochem., 63, 2168-2173.
    View publication on PubMed

Application

Application Comment Organism
synthesis production of D-glutamate from L-glutamate with glutamate racemase and L-glutamate oxidase from Streptomyces sp. X119-6. Both enzymes are highly stabilized by immobilization Bacillus subtilis

General Stability

General Stability Organism
stabilized by immobilization to Chitopearl 2505 or Chitopearl 2605. The enzyme remains active at 4°C for 30 days, remaining activity is 32% Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
IFO 3336
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
soluble and immobilized enzyme Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 20°C: about 65% of maximal activity, 70°C: about 30% of maximal activity, soluble enzyme Bacillus subtilis
20 70 more than 80% of maximal activity is observed between 20°C and 70°C, immobilized enzyme Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
pH 7, 60 min, about 25% loss of activity of soluble and immobilized enzyme Bacillus subtilis
80
-
pH 7, 60 min, about 20% loss of activity of immobilized enzyme, about 70% loss of activity of soluble enzyme Bacillus subtilis
90
-
pH 7, 60 min, 50% loss of activity of immobilized enzyme, about 75% loss of activity of soluble enzyme Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
soluble and immobilized enzyme Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
2 10 pH 2.0: about 40% of maximal activity of soluble enzyme, about 60% of maximal activity of immobilized enzyme, pH 10.0: about 80% of maximal activity of immobilized enzyme, about 40% of maximal activity of soluble enzyme Bacillus subtilis