Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 40% residual activity at 1 mM | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.34 | - |
L-alanine | at pH 9.0 and 37°C | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activation at 1 mM | Pseudomonas putida | |
Co2+ | 120% activity at 1 mM | Pseudomonas putida | |
Fe2+ | slight activation at 1 mM | Pseudomonas putida | |
Mg2+ | slight activation at 1 mM | Pseudomonas putida | |
Mn2+ | 130% activity at 1 mM | Pseudomonas putida | |
additional information | not influenced by 1 mM Zn2+ or Sn2+ | Pseudomonas putida | |
Ni2+ | slight activation at 1 mM | Pseudomonas putida | |
Sr2+ | 158% activity at 1 mM | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42370 | - |
gel filtration | Pseudomonas putida |
43000 | - |
1 * 43000, SDS-PAGE | Pseudomonas putida |
44217 | - |
1 * 44217, calculated from amino acid sequence | Pseudomonas putida |
44220 | - |
calculated from amino acid sequence | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida YZ-26 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography and Sephacryl S-200 gel filtration | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.5 | - |
crude extract, using L-alanine as substrate, at pH 9.0 and 37°C | Pseudomonas putida |
16.5 | - |
after 3.7fold purification, using L-isoleucine as substrate, at pH 9.0 and 37°C | Pseudomonas putida |
42.6 | - |
after 3.7fold purification, using L-alanine as substrate, at pH 9.0 and 37°C | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | optimal substrate for alanine racemase | Pseudomonas putida | D-alanine | - |
? | |
L-alanine | optimal substrate for alanine racemase | Pseudomonas putida YZ-26 | D-alanine | - |
? | |
L-isoleucine | - |
Pseudomonas putida | D-isoleucine | - |
? | |
L-isoleucine | - |
Pseudomonas putida YZ-26 | D-isoleucine | - |
? | |
additional information | the enzyme is only specific to L-alanine and L-isoleucine, and does not catalyze isomerization the other amino acids | Pseudomonas putida | ? | - |
? | |
additional information | the enzyme is only specific to L-alanine and L-isoleucine, and does not catalyze isomerization the other amino acids | Pseudomonas putida YZ-26 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, SDS-PAGE | Pseudomonas putida |
monomer | 1 * 44217, calculated from amino acid sequence | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
ALR | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | - |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 80 | 95% activity at 25°C, 80% activity at 40°C, less than 20% activity at 50°C, less than 10% activity at 60 and 70°C, inactive at 80°C | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70.63 | - |
L-alanine | at pH 9.0 and 37°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | when the enzyme is incubated at 37°C in 100 mM sodium acetate, 100 mM sodium phosphate, 100 mM Tris/HCl, and 100 mM borate/NaOH buffer for 30 min, more than 80% of the initial activity is retained at pH 7.0-11.0 | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas putida |