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Literature summary for 5.1.1.1 extracted from
- Expression, purification, and characterization of alanine racemase from Pseudomonas putida YZ-26 (2012), World J. Microbiol. Biotechnol., 28, 267-274.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 40% residual activity at 1 mM | Pseudomonas putida |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.34 | - |
L-alanine | at pH 9.0 and 37°C | Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight activation at 1 mM | Pseudomonas putida | |
| Co2+ | 120% activity at 1 mM | Pseudomonas putida | |
| Fe2+ | slight activation at 1 mM | Pseudomonas putida | |
| Mg2+ | slight activation at 1 mM | Pseudomonas putida | |
| Mn2+ | 130% activity at 1 mM | Pseudomonas putida | |
| additional information | not influenced by 1 mM Zn2+ or Sn2+ | Pseudomonas putida | |
| Ni2+ | slight activation at 1 mM | Pseudomonas putida | |
| Sr2+ | 158% activity at 1 mM | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42370 | - |
gel filtration | Pseudomonas putida |
| 43000 | - |
1 * 43000, SDS-PAGE | Pseudomonas putida |
| 44217 | - |
1 * 44217, calculated from amino acid sequence | Pseudomonas putida |
| 44220 | - |
calculated from amino acid sequence | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida YZ-26 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA column chromatography and Sephacryl S-200 gel filtration | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.5 | - |
crude extract, using L-alanine as substrate, at pH 9.0 and 37°C | Pseudomonas putida |
| 16.5 | - |
after 3.7fold purification, using L-isoleucine as substrate, at pH 9.0 and 37°C | Pseudomonas putida |
| 42.6 | - |
after 3.7fold purification, using L-alanine as substrate, at pH 9.0 and 37°C | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-alanine | optimal substrate for alanine racemase | Pseudomonas putida | D-alanine | - |
? | |
| L-alanine | optimal substrate for alanine racemase | Pseudomonas putida YZ-26 | D-alanine | - |
? | |
| L-isoleucine | - |
Pseudomonas putida | D-isoleucine | - |
? | |
| L-isoleucine | - |
Pseudomonas putida YZ-26 | D-isoleucine | - |
? | |
| additional information | the enzyme is only specific to L-alanine and L-isoleucine, and does not catalyze isomerization the other amino acids | Pseudomonas putida | ? | - |
? | |
| additional information | the enzyme is only specific to L-alanine and L-isoleucine, and does not catalyze isomerization the other amino acids | Pseudomonas putida YZ-26 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Pseudomonas putida |
| monomer | 1 * 44217, calculated from amino acid sequence | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALR | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Pseudomonas putida |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | - |
Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 80 | 95% activity at 25°C, 80% activity at 40°C, less than 20% activity at 50°C, less than 10% activity at 60 and 70°C, inactive at 80°C | Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 70.63 | - |
L-alanine | at pH 9.0 and 37°C | Pseudomonas putida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | when the enzyme is incubated at 37°C in 100 mM sodium acetate, 100 mM sodium phosphate, 100 mM Tris/HCl, and 100 mM borate/NaOH buffer for 30 min, more than 80% of the initial activity is retained at pH 7.0-11.0 | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Pseudomonas putida | |
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