Application | Comment | Organism |
---|---|---|
medicine | since it is absent in humans, this enzyme is an attractive target for the development of drugs against Streptococcus pneumoniae and other bacterial pathogens | Streptococcus pneumoniae |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, at 4°C in 1.2 M sodium citrate, 0.1 M MES, pH 7.2, and 10% (v/v) glycerol | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | P0A2W8 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, anion-exchange chromatography, hydrophobic interaction chromatography, and gel filtration | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | - |
Streptococcus pneumoniae | D-alanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
ALR | - |
Streptococcus pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Streptococcus pneumoniae |