Application | Comment | Organism |
---|---|---|
pharmacology | potential candidate for the development of a recombinant vaccine | Bacillus anthracis |
Cloned (Comment) | Organism |
---|---|
expressed as recombinant monomeric protein in Escherichia coli | Bacillus anthracis |
Protein Variants | Comment | Organism |
---|---|---|
D48A | no effect on the enzyme activity | Bacillus anthracis |
K41A | completely inactive | Bacillus anthracis |
Y270A | impaired enzyme activity | Bacillus anthracis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | non-competitive inhibition kinetics | Bacillus anthracis | |
propionate | propionate influences both Km (affinity for substrate) and Vmax (enzyme catalysis) | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
L-alanine | - |
Bacillus anthracis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Bacillus anthracis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | Bacillus anthracis | - |
D-alanine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus anthracis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spore | - |
Bacillus anthracis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
295 | - |
at 37°C | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | - |
Bacillus anthracis | D-alanine | - |
r | |
L-leucine | activity is 20% compared with L-alanine, other amino acids are not racemized | Bacillus anthracis | D-leucine | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | gel filtration | Bacillus anthracis |
Synonyms | Comment | Organism |
---|---|---|
alanine racemase | - |
Bacillus anthracis |
ALR | - |
Bacillus anthracis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus anthracis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | - |
Bacillus anthracis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 85 | between 60 and 70°C 50% of the enzyme activity is lost, at 85°C 50% of the protein molecules are denatured, the residual activity is 4% | Bacillus anthracis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
L-alanine | - |
Bacillus anthracis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bacillus anthracis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Bacillus anthracis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP is inherently bound to the enzyme, removal of PLP inactivates the enzyme, adding PLP restores the activity, addition of 10 microM PLP to native enzyme slightly enhances activity | Bacillus anthracis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
hydroxylamine | - |
Bacillus anthracis | |
30 | - |
propionate | - |
Bacillus anthracis |
General Information | Comment | Organism |
---|---|---|
metabolism | plays a role in spore germination and cell wall biosynthesis | Bacillus anthracis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.33 | - |
L-alanine | - |
Bacillus anthracis |