Literature summary for 5.1.1.1 extracted from

Priyadarshi, A.; Lee, E.H.; Sung, M.W.; Kim, J.H.; Ku, M.J.; Kim, E.E.; Hwang, K.Y.
Overexpression, crystallization, and preliminary X-ray crystallographic analysis of the alanine racemase from Enterococcus faecalis v583 (2008), J. Microbiol. Biotechnol., 18, 55-58.

Cloned(Commentary)

Cloned (Comment)	Organism
amplified from genomic DNA from Enterococcus faecalis via PCR, integrated into pET22b, transformation of Escherichia coli BL21 (DE3) for overexpression as His-tagged protein	Enterococcus faecalis

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor-diffusion method, protein concentration: 20 mg/ml; crystal screen buffer: 0.1 M HEPES, pH 8.0, 22% (w/v) PEG 8000, 0.3 M Ca-acetate, and 1/10 (v/v) cyclohexyl-methyl-beta-D-maltoside, drop: ratio 1/2 mixed protein solution and buffer (sum 2 microl) hanging over 1 ml of buffer; cryo-protecting solution: 0.1 M HEPES, pH 8.0, 22% (w/v) PEG 8000, 0.3 M Ca-acetate, 30% (v/v) glycerol and 1/10 (v/v) cyclohexyl-methyl-beta-D-maltoside for flashcooling in liquid nitrogen, diffracted to 2.5 A	Enterococcus faecalis

Crystallization (Comment)

Organism

hanging-drop vapor-diffusion method, protein concentration: 20 mg/ml; crystal screen buffer: 0.1 M HEPES, pH 8.0, 22% (w/v) PEG 8000, 0.3 M Ca-acetate, and 1/10 (v/v) cyclohexyl-methyl-beta-D-maltoside, drop: ratio 1/2 mixed protein solution and buffer (sum 2 microl) hanging over 1 ml of buffer; cryo-protecting solution: 0.1 M HEPES, pH 8.0, 22% (w/v) PEG 8000, 0.3 M Ca-acetate, 30% (v/v) glycerol and 1/10 (v/v) cyclohexyl-methyl-beta-D-maltoside for flashcooling in liquid nitrogen, diffracted to 2.5 A

Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40100	-	SDS-PAGE	Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-alanine	Enterococcus faecalis	-	D-alanine	-	r

Organism

Organism	UniProt	Comment	Textmining
Enterococcus faecalis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA resin (His-tagged overexpressed protein), Hitrap Q-column, Superdex S prep grad column	Enterococcus faecalis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-alanine	-	Enterococcus faecalis	D-alanine	-	r

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Enterococcus faecalis