Literature summary for 5.1.1.1 extracted from

Chesnokova, O.N.; McPherson, S.A.; Steichen, C.T.; Turnbough, C.L.
The spore-specific alanine racemase of Bacillus anthracis and its role in suppressing germination during spore development (2009), J. Bacteriol., 191, 1303-1310.

Cloned(Commentary)

Cloned (Comment)	Organism
analyzation of alanine racemase-transcripts (RT-PCR) during growth and sporulation of wild-type shows low transcription level during early and higher during late sporulation states, analyzed expression level (immunoblotting) shows only alanine racemase during late sporulating states, growth rate of alr-gene knockout mutant and wild-type are identical, mutant only produces half as many spores as wild-type, mutant and wild-type show same resistance against heat, lysozyme and organic solvents, germination takes place in mutant at lower levels of L-alanine (a suggested germination activator) than in wild-type (D-alanine ist suggested to inhibit germination when unfavourable growth conditions), conversion of phase-bright spores to phase-dark germinating cells takes already place within mother cells of the alr mutant (phase dark to phase light), not in wild-type cells resulting in non-resistant mutant-germinants and resistant wild-type-spores respectively	Bacillus anthracis

Cloned (Comment)

Organism

analyzation of alanine racemase-transcripts (RT-PCR) during growth and sporulation of wild-type shows low transcription level during early and higher during late sporulation states, analyzed expression level (immunoblotting) shows only alanine racemase during late sporulating states, growth rate of alr-gene knockout mutant and wild-type are identical, mutant only produces half as many spores as wild-type, mutant and wild-type show same resistance against heat, lysozyme and organic solvents, germination takes place in mutant at lower levels of L-alanine (a suggested germination activator) than in wild-type (D-alanine ist suggested to inhibit germination when unfavourable growth conditions), conversion of phase-bright spores to phase-dark germinating cells takes already place within mother cells of the alr mutant (phase dark to phase light), not in wild-type cells resulting in non-resistant mutant-germinants and resistant wild-type-spores respectively

Bacillus anthracis

Protein Variants

Protein Variants	Comment	Organism
deltaalr	mutant with knocked out alanine racemase gene alr (2 alanine racemase-genes have been found in Bacillus anthracis jet)	Bacillus anthracis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43660	-	immunoblotting	Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-alanine	Bacillus anthracis	-	D-alanine	-	r

Organism

Organism	UniProt	Comment	Textmining
Bacillus anthracis	Q81VF6	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
spore	-	Bacillus anthracis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-alanine	-	Bacillus anthracis	D-alanine	-	r

Synonyms

Synonyms	Comment	Organism
BAS0238	-	Bacillus anthracis

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Bacillus anthracis