Cloned (Comment) | Organism |
---|---|
amplified by PCR, cloned and overexpressed with pOPINB in Escherichia coli Rosetta pLysS cells, His-tagged product | Bacillus anthracis |
Crystallization (Comment) | Organism |
---|---|
enzyme is subjected to a reductive-methylation procedure | Bacillus anthracis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-1-aminoethylphosphonic acid | in combination with pyridoxal 5'-phosphate | Bacillus anthracis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
exosporium | - |
Bacillus anthracis | 43592 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43810 | - |
mass-spectrometry | Bacillus anthracis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | Bacillus anthracis | - |
D-alanine | enzyme provides D-Ala as a required compound for the synthesis of the peptidoglycan layer of the bacterial cell wall, Tolypocladium niveum requires alanine racemase for cyclosporin biosynthesis | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | Q81VF6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | - |
Bacillus anthracis | D-alanine | enzyme provides D-Ala as a required compound for the synthesis of the peptidoglycan layer of the bacterial cell wall, Tolypocladium niveum requires alanine racemase for cyclosporin biosynthesis | r |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallography | Bacillus anthracis |
Synonyms | Comment | Organism |
---|---|---|
BA0252 | - |
Bacillus anthracis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | C-terminal region of 1 subdomain: Arg138 donates a hydrogen bond to the phenolic O atom of PLP, Arg224 donates a hydrogen bond to the pyridinyl N atom of PLP, Lys41 forms an aldimine linkage with the PLP, eliminating water to form the Schiff base, C-terminal atoms of second subunit Ser209, Gly226 and Ile227 stabilize the PLP phosphate with the help of Ser209 O(gamma), Tyr45 O(eta) and Tyr359 O(eta) | Bacillus anthracis |