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Literature summary for 5.1.1.1 extracted from

  • Fenn, T.D.; Holyoak, T.; Stamper, G.F.; Ringe, D.
    Effect of a Y265F mutant on the transamination-based cycloserine inactivation of alanine racemase (2005), Biochemistry, 44, 5317-5327.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant Y265F in complex with D- and with L-cycloserine Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
Y265F 1600fold reduction of racemization Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
D-cycloserine model for inactivation mechanism via geminal diamine and ketimine to isoxazole Geobacillus stearothermophilus
L-Cycloserine model for inactivation mechanism via geminal diamine and ketimine to isoxazole Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus P10724
-
-

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.33
-
D-cycloserine mutant Y265F, 37°C, pH 9.1 Geobacillus stearothermophilus
4
-
D-cycloserine or higher, wild-type, 37°C, pH 9.1 Geobacillus stearothermophilus
6
-
L-Cycloserine wild-type, 37°C, pH 9.1 Geobacillus stearothermophilus