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Literature summary for 5.1.1.1 extracted from

  • Noda, M.; Matoba, Y.; Kumagai, T.; Sugiyama, M.
    A novel assay method for an amino acid racemase reaction based on circular dichroism (2005), Biochem. J., 389, 491-496.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 D-alanine pH 8.2, 37°C Escherichia coli
0.29
-
 L-alanine pH 8.2, 37°C Escherichia coli
0.4
-
 L-alanine pH 8.2, 37°C Streptomyces lavendulae
0.4
-
 D-alanine pH 8.2, 37°C Streptomyces lavendulae
10
-
 D-serine pH 8.2, 37°C Streptomyces lavendulae
27
-
 L-serine pH 8.2, 37°C Streptomyces lavendulae

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Streptomyces lavendulae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 assay method based on circular dichroism spectra of D- and L-alanine Escherichia coli
additional information
-
 assay method based on circular dichroism spectra of D- and L-alanine Streptomyces lavendulae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine
-
 Escherichia coli L-alanine
-
 r
D-alanine
-
 Streptomyces lavendulae L-alanine
-
 r
D-serine
-
 Streptomyces lavendulae L-serine
-
 r
L-alanine
-
 Escherichia coli D-alanine
-
 r
L-alanine
-
 Streptomyces lavendulae D-alanine
-
 r
L-serine
-
 Streptomyces lavendulae D-serine
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 D-alanine pH 8.2, 37°C Escherichia coli
5.5
-
 D-serine pH 8.2, 37°C Streptomyces lavendulae
13.3
-
 L-serine pH 8.2, 37°C Streptomyces lavendulae
27.6
-
 L-alanine pH 8.2, 37°C Escherichia coli
55
-
 L-alanine pH 8.2, 37°C Streptomyces lavendulae
63.3
-
 D-alanine pH 8.2, 37°C Streptomyces lavendulae