Literature summary for 5.1.1.1 extracted from

Strych, U.; Penland, R.L.; Jimenez, M.; Krause, K.L.; Benedik, M.J.
Characterization of the alanine racemases from two mycobacteria (2001), FEMS Microbiol. Lett., 196, 93-98.

Search for more literature for 5.1.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
amplified by PCR, cloned and overexpressed in Escherichia coli BL21, both as native and His-tagged products	Mycobacterium avium
amplified by PCR, cloned and overexpressed in Escherichia coli BL21, both as native and His-tagged products	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
cycloserine	D-cycloserine or a racemic mixture of D- and L-cycloserine	Mycobacterium avium
cycloserine	D-cycloserine or a racemic mixture of D- and L-cycloserine	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.4	-	D-Ala	-	Mycobacterium avium
0.5	-	L-Ala	-	Mycobacterium avium
1.1	-	D-Ala	23°C	Mycobacterium tuberculosis
1.2	-	L-Ala	23°C	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium avium	-	-	-
Mycobacterium tuberculosis	P9WQA9	-	-
Mycobacterium tuberculosis H37Rv	P9WQA9	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mycobacterium avium
-	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Ala	-	Mycobacterium avium	D-Ala	-	r
L-Ala	-	Mycobacterium tuberculosis	D-Ala	-	r
L-Ala	-	Mycobacterium tuberculosis H37Rv	D-Ala	-	r

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Release 2023.1 (January 2023)