Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli SOLR with a plasmid yYOK3 | Peribacillus psychrosaccharolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.2 | - |
D-Ala | 30°C, pH 8.3 | Peribacillus psychrosaccharolyticus | |
17.9 | - |
L-Ala | 30°C, pH 8.3 | Peribacillus psychrosaccharolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42500 | - |
2 * 42500, SDS-PAGE | Peribacillus psychrosaccharolyticus |
73000 | - |
gel filtration | Peribacillus psychrosaccharolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Peribacillus psychrosaccharolyticus | Q9S5V6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Peribacillus psychrosaccharolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
650 | - |
L-Ala, 0°C | Peribacillus psychrosaccharolyticus |
Storage Stability | Organism |
---|---|
-80°C, 60 days | Peribacillus psychrosaccharolyticus |
4°C, 3 weeks | Peribacillus psychrosaccharolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | - |
Peribacillus psychrosaccharolyticus | D-Ala | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42500, SDS-PAGE | Peribacillus psychrosaccharolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
in absence of pyridoxal 5'-phosphate | Peribacillus psychrosaccharolyticus |
60 | - |
in presence of an excess amount of pyridoxal 5'-phosphate, 0.4 mM | Peribacillus psychrosaccharolyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
60 | about 30% of maximal activity at 0°C and at 60°C, in absence of pyridoxal 5'-phosphate | Peribacillus psychrosaccharolyticus |
30 | 50 | 30°C, 45% of maximal activity, 50°C: about 50% of maximal activity, in presence of an excess amount of pyridoxal 5'-phosphate, 0.4 mM | Peribacillus psychrosaccharolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
pH 8.3, 1 h, the purified is extremely labile over 35°C | Peribacillus psychrosaccharolyticus |
50 | - |
pH 8.3, 1 h, stabilized up to 50°C in presence of excess amounts of pyridoxal 5'-phosphate, 0.4 mM, complete inactivation in absence of pyridoxal 5'-phosphate | Peribacillus psychrosaccharolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | - |
Peribacillus psychrosaccharolyticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | Km: 0.005 mM, at 30°C | Peribacillus psychrosaccharolyticus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Peribacillus psychrosaccharolyticus | - |
- |
5.7 |