KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.8 | - |
D-Ala | - |
Pseudomonas fluorescens | |
18.9 | - |
L-Ala | - |
Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Pseudomonas fluorescens |
76000 | - |
gel filtration in presence or absence of 2-mercaptoethanol | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
587 | - |
- |
Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
-20°C, stable for 1 month | Pseudomonas fluorescens |
4°C, stable for 1 week | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | - |
Pseudomonas fluorescens | D-Ala | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas fluorescens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
40 | 0°C: about 25% of maximal activity, 40°C: about 45% of maximal activity | Pseudomonas fluorescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
1 h, stable | Pseudomonas fluorescens |
30 | - |
over 30°C, 1 h, quick loss of activity | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 2 mol of pyridoxal 5'-phosphate bound per mol of enzyme dimer | Pseudomonas fluorescens |