Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | - |
Bacillus thuringiensis |
Cloned (Comment) | Organism |
---|---|
transformed into Escherichia coli BL21-Codonplus (DE3)-RIL cells | Bacillus thuringiensis |
Protein Variants | Comment | Organism |
---|---|---|
G48A | single mutant | Bacillus thuringiensis |
I43A | single mutant | Bacillus thuringiensis |
I43W | single mutant | Bacillus thuringiensis |
I43W/W47I | are made by introducing the second mutation in the gene coding for a single mutant | Bacillus thuringiensis |
K44E | single mutant | Bacillus thuringiensis |
L39A | single mutant | Bacillus thuringiensis |
L39A/V46A | are made by introducing the second mutation in the gene coding for a single mutant | Bacillus thuringiensis |
P42G | single mutant | Bacillus thuringiensis |
Q45A | single mutant | Bacillus thuringiensis |
V46A | single mutant | Bacillus thuringiensis |
W47A/W242A | dimeric mutant, which is unable to bind to phosphatidycholine | Bacillus thuringiensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | P08954 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-sepharose fast-flow column and phenyl-Sepharose column, monitored by SDS-PAGE | Bacillus thuringiensis |
Subunits | Comment | Organism |
---|---|---|
monomer | although small amounts of dimer have been noted previously | Bacillus thuringiensis |
Synonyms | Comment | Organism |
---|---|---|
phosphatidylinositol-specific phospholipase C | - |
Bacillus thuringiensis |
PI-PLC | - |
Bacillus thuringiensis |