Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Isopropanol | 30%, activates | Bacillus thuringiensis | |
additional information | PI-PLC exhibits several types of kinetic interfacial activation by interfaces, roles of Trp-47 and Trp-242 | Bacillus thuringiensis | |
phosphatidylcholine | activates | Bacillus thuringiensis |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21 | Bacillus thuringiensis |
Protein Variants | Comment | Organism |
---|---|---|
W178A | mutant with reduced stability and specific activity, study of kinetic activation by micellar phosphatidylcholine | Bacillus thuringiensis |
W242A | active enzyme, partitioning of mutant enzyme to vesicles is decreased by more than 10fold, study of kinetic activation by micellar phosphatidylcholine | Bacillus thuringiensis |
W242F | kinetic analysis, binding studies to phosphatidylcholine vesicles | Bacillus thuringiensis |
W242I | kinetic analysis, binding studies to phosphatidylcholine vesicles | Bacillus thuringiensis |
W280A | mutant with reduced stability, study of kinetic activation by micellar phosphatidylcholine | Bacillus thuringiensis |
W47A | active enzyme, partitioning of mutant enzyme to vesicles is decreased by more than 10fold, study of kinetic activation by micellar phosphatidylcholine | Bacillus thuringiensis |
W47A/W242A | double mutant, no affinity for phospholipid surfaces, no kinetic activation by micellar phosphatidylcholine | Bacillus thuringiensis |
W47F | kinetic analysis, binding studies to phosphatidylcholine vesicles | Bacillus thuringiensis |
W47I | kinetic analysis, binding studies to phosphatidylcholine vesicles | Bacillus thuringiensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
myo-inositol | poor competitive inhibitor | Bacillus thuringiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Bacillus thuringiensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | recombinant PI-PLC, expressed in Escherichia coli BL21 | Bacillus thuringiensis | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activities of wild-type and mutant PI-PLCs towards 1D-myo-inositol 1,2-cyclic phosphate in the absence and presence of different activators | Bacillus thuringiensis |
401 | - |
W47I mutant PI-PLC, phosphotransferase activity | Bacillus thuringiensis |
556 | - |
wild-type PI-PLC, phosphotransferase activity | Bacillus thuringiensis |
558 | - |
W47F mutant PI-PLC, phosphotransferase activity | Bacillus thuringiensis |
658 | - |
W242F mutant PI-PLC, phosphotransferase activity | Bacillus thuringiensis |
684 | - |
W242I mutant PI-PLC, phosphotransferase activity | Bacillus thuringiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol | Trp-47 and Trp-242 residues are important for enzyme to bind to interfaces, both activating zwitterionic and substrate anionic surfaces, micellar phosphatidylinositol is a better substrate than monomeric phosphatidylinositol | Bacillus thuringiensis | 1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol | a cyclic phosphodiesterase activity of PI-PLC converts 1D-myo-inositol 1,2-cyclic phosphate to inositol 1-phosphate | ? | |
additional information | not: phosphatidylcholine | Bacillus thuringiensis | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
41.5 | - |
Tm-value, pH 8, W178A mutant PI-PLC | Bacillus thuringiensis |
47 | - |
Tm-value, pH 8, W280A mutant PI-PLC | Bacillus thuringiensis |
53.6 | - |
Tm-value, pH 8, W47A mutant PI-PLC | Bacillus thuringiensis |
54.4 | - |
Tm-value, pH 8, wild-type PI-PLC | Bacillus thuringiensis |
54.6 | - |
Tm-value, pH 8, W47A/W242A double mutant PI-PLC | Bacillus thuringiensis |
56.2 | - |
Tm-value, pH 8, W242A mutant PI-PLC | Bacillus thuringiensis |