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Literature summary for 4.6.1.13 extracted from

  • Kravchuk, A.V.; Zhao, L.; Kubiak, R.J.; Bruzik, K.S.; Tsai, M.D.
    Mechanism of phosphatidylinositol-specific phospholipase C: Origin of unusually high nonbridging thio effects (2001), Biochemistry, 40, 5433-5439.
    View publication on PubMed
Search for more literature for 4.6.1.13

Protein Variants

Protein Variants Comment Organism
R69A mutant is specifically activated by guanidinium hydrochloride Bacillus sp. (in: Bacteria)
R69C site-directed chemical modification of the cysteine residue replacing Arg-69, mutant PI-PLCs featuring bidentate side chains at this position display significantly higher activity, higher thio effects, and greater stereoselectivity than do those with monodentate side chains Bacillus sp. (in: Bacteria)
R69G mutant is specifically activated by guanidinium hydrochloride Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34460
-
 R69C mutant PI-PLC, electrospray ionization mass spectrometry Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
R69C mutant PI-PLC Bacillus sp. (in: Bacteria)

Reaction

Reaction Comment Organism Reaction ID
1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol mechanism Bacillus sp. (in: Bacteria)
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol catalytic mechanism, role of Arg-69, the bidentate nature of Arg-69 is the origin of the large thio effects and stereoselectivity in PI-PLC, its function is to bring the phosphate group and the 2-OH group of inositol into proximity and to induce proper alignment for nucleophilic attack, and possibly to lower the pKa of the 2-OH Bacillus sp. (in: Bacteria) 1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Bacillus sp. (in: Bacteria)