Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.1 extracted from

  • Kleinboelting, S.; Diaz, A.; Moniot, S.; van den Heuvel, J.; Weyand, M.; Levin, L.R.; Buck, J.; Steegborn, C.
    Crystal structures of human soluble adenylyl cyclase reveal mechanisms of catalysis and of its activation through bicarbonate (2014), Proc. Natl. Acad. Sci. USA, 111, 3727-3732.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.1

Activating Compound

Activating Compound Comment Organism Structure
HCO3- the enzyme is directly activated by bicarbonate Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli in Hi5 cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 100 mM sodium acetate (pH 4.8), 200 mM trisodium-citrate, 18% (w/v) PEG 4000, 20% (v/v) glycerol Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid
-
 Homo sapiens
GTP
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP Homo sapiens
-
 3',5'-cyclic AMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
 Homo sapiens 3',5'-cyclic AMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
sAC
-
 Homo sapiens
soluble adenylyl cyclase
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
 GTP in 50 mM Tris-HCl (pH 8.0), 50 mM NaCl, 10 mM MgCl2, 10 mM CaCl2, at 37°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.043
-
 in 50 mM Tris-HCl (pH 8.0), 50 mM NaCl, 10 mM MgCl2, 10 mM CaCl2, at 37°C Homo sapiens 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid