Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli SG13009[pREP4] andM15[pREP4] cells | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
using 0.1 M 4-morpholineethanesulfonic acid (pH 6.5) and 12% (w/v) polyethylene glycol 20000 | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
A197T | the mutant shows increased activity compared to the wild type enzyme | Pseudomonas aeruginosa |
E189R | the mutant shows increased activity compared to the wild type enzyme | Pseudomonas aeruginosa |
E377G | the mutant shows increased activity compared to the wild type enzyme | Pseudomonas aeruginosa |
F399H | the mutant shows increased activity compared to the wild type enzyme | Pseudomonas aeruginosa |
F399I | the mutant shows increased activity compared to the wild type enzyme | Pseudomonas aeruginosa |
I352T | the mutant shows increased activity compared to the wild type enzyme | Pseudomonas aeruginosa |
L326P | the mutant shows increased activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R318W | the mutant shows increased activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R412H | the mutant shows slightly increased activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R456L | the mutant shows strongly increased activity compared to the wild type enzyme | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-hydroxyestradiol | significant inhibition at 0.1 mM | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inner membrane | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | Pseudomonas aeruginosa | - |
3',5'-cyclic AMP + diphosphate | - |
? | |
ATP | Pseudomonas aeruginosa ATCC 15692 | - |
3',5'-cyclic AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HZ23 | - |
- |
Pseudomonas aeruginosa ATCC 15692 | Q9HZ23 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Superose 12 gel filtration | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | - |
Pseudomonas aeruginosa | 3',5'-cyclic AMP + diphosphate | - |
? | |
ATP | - |
Pseudomonas aeruginosa ATCC 15692 | 3',5'-cyclic AMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 40000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
adenylyl cyclase | - |
Pseudomonas aeruginosa |
CyaB | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is a key regulator of Pseudomonas aeruginosa virulence | Pseudomonas aeruginosa |