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Literature summary for 4.6.1.1 extracted from

  • Suryanarayana, S.; Goettle, M.; Huebner, M.; Gille, A.; Mou, T.C.; Sprang, S.R.; Richter, M.; Seifert, R.
    Differential inhibition of various adenylyl cyclase isoforms and soluble guanylyl cyclase by 2,3-O-(2,4,6-trinitrophenyl)-substituted nucleoside 5-triphosphates (2009), J. Pharmacol. Exp. Ther., 330, 687-695.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2',3'-O-(2,4,6-trinitrophenyl)-ADP
-
 Homo sapiens
2',3'-O-(2,4,6-trinitrophenyl)-AMP
-
 Homo sapiens
2',3'-O-(2,4,6-trinitrophenyl)-ATP most potent inhibitor for isoform ACV Homo sapiens
2',3'-O-(2,4,6-trinitrophenyl)-CTP
-
 Homo sapiens
2',3'-O-(2,4,6-trinitrophenyl)-GDP
-
 Homo sapiens
2',3'-O-(2,4,6-trinitrophenyl)-GTP
-
 Homo sapiens
2',3'-O-(2,4,6-trinitrophenyl)-UTP most potent inhibitor for isoforms ACI and ACII Homo sapiens
additional information 2',3'-O-(2,4,6-trinitrophenyl)-nucleotides (TNP-nucleotides) inhibit isoform ACI in the order of potency TNP-UTP=TNP-ATP>TNP-GTP=TNP-CTP>TNP-ADP>TNP-GDP>>>TNP-AMP. Specifically, the order of potency for isoform ACII is TNP-UTP >TNP-ATP=TNP-CTP >TNP-GTP >>TNP-ADP>TNP-GDP > TNP-AMP. Specifically, TNP-nucleotides inhibit isoform ACV in the order of potency: TNP-ATP>TNP-UTP>TNP-GTP=TNP-CTP>>TNP-ADP>TNP-GDP>TNP-AMP Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP Homo sapiens
-
 3',5'-cyclic-AMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose 4B resin column chromatography, Q-Sepharose column chromatography, and Talon resin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
 Homo sapiens 3',5'-cyclic-AMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
ACI isoform Homo sapiens
ACII isoform Homo sapiens
ACV isoform Homo sapiens
adenylyl cyclase
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000037
-
 2',3'-O-(2,4,6-trinitrophenyl)-ATP isoform ACV, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.0000071
-
 2',3'-O-(2,4,6-trinitrophenyl)-UTP isoform ACI, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.000009
-
 2',3'-O-(2,4,6-trinitrophenyl)-ATP isoform ACI, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.000015
-
 2',3'-O-(2,4,6-trinitrophenyl)-UTP isoform ACV, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.000023
-
 2',3'-O-(2,4,6-trinitrophenyl)-GTP isoform ACI, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.000024
-
 2',3'-O-(2,4,6-trinitrophenyl)-CTP isoform ACI, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.000024
-
 2',3'-O-(2,4,6-trinitrophenyl)-UTP isoform ACII, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.000027
-
 2',3'-O-(2,4,6-trinitrophenyl)-GTP isoform ACV, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.000031
-
 2',3'-O-(2,4,6-trinitrophenyl)-CTP isoform ACV, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.000099
-
 2',3'-O-(2,4,6-trinitrophenyl)-ATP isoform ACII, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.00011
-
 2',3'-O-(2,4,6-trinitrophenyl)-CTP isoform ACII, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.00014
-
 2',3'-O-(2,4,6-trinitrophenyl)-ADP isoform ACI, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.00022
-
 2',3'-O-(2,4,6-trinitrophenyl)-GTP isoform ACII, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.00037
-
 2',3'-O-(2,4,6-trinitrophenyl)-ADP isoform ACV, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.00041
-
 2',3'-O-(2,4,6-trinitrophenyl)-GDP isoform ACI, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.0011
-
 2',3'-O-(2,4,6-trinitrophenyl)-ADP isoform ACII, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.0012
-
 2',3'-O-(2,4,6-trinitrophenyl)-GDP isoform ACV, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.0034
-
 2',3'-O-(2,4,6-trinitrophenyl)-GDP isoform ACII, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.0048
-
 2',3'-O-(2,4,6-trinitrophenyl)-AMP isoform ACI, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.0048
-
 2',3'-O-(2,4,6-trinitrophenyl)-AMP isoform ACV, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens
0.0074
-
 2',3'-O-(2,4,6-trinitrophenyl)-AMP isoform ACII, in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C Homo sapiens