Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | a second isoform- and regulator-specific contact site in C2 is necessary to render enzyme activity susceptible to calmodulin modulation. In addition to the PFAHL-motif in C1b of ACII, calmodulin requires not only the Ca2+-independent AC28-region in C1b but also a Ca2+-dependent domain in C2a of ACI with the VLG-loop to stimulate this adenylyl cyclase isoform | Bos taurus | |
forskolin | - |
Bos taurus | |
Galphas | - |
Bos taurus | |
Gbetagamma | a second isoform- and regulator-specific contact site in C2 is necessary to render enzyme activity susceptible to Gbetagamma modulation. In addition to the PFAHL-motif in C1b of ACII, Gbetagamma contacts the KF-loop in C2 | Bos taurus |
Cloned (Comment) | Organism |
---|---|
expressed in Sf9 cells | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | ACI-deletion mutants ACI.lambda1057 with reduced catalytic activity and ACI.lambda1094, which like mutant ACIlambda1057 is active and stimulated by Ca/CaM as well as ACI. Mutant ACII.lambda928 is catalytically inactive, mutant ACII.AA930 shows diminished activity, mutants ACII.AA925 or ACII.AA932 show no significant change in Gbetagamma-regulation | Bos taurus |
V1027A/L1031A | is not catalytically active under basal, Galphas- or forskolin-stimulated conditions | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P19754 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | - |
Bos taurus | 3',5'-cAMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACI | - |
Bos taurus |
ACII | - |
Bos taurus |