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Literature summary for 4.6.1.1 extracted from

  • Masuda, S.; Ono, T.a.
    Biochemical characterization of the major adenylyl cyclase, Cya1, in the cyanobacterium Synechocystis sp. PCC 6803 (2004), FEBS Lett., 577, 255-258.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Synechocystis sp. PCC 6803

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
ATP pH 7.5, 27°C Synechocystis sp. PCC 6803

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ about 50fold lower enzyme activity than in the presence of Mn2+ Synechocystis sp. PCC 6803
Mn2+
-
Synechocystis sp. PCC 6803

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Synechocystis sp. PCC 6803

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Synechocystis sp. PCC 6803

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
Vmax 66.3 pmol cAMP/min/nmol, ph 7.5, 27°C Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
Synechocystis sp. PCC 6803 3',5'-cAMP + diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Synechocystis sp. PCC 6803

Synonyms

Synonyms Comment Organism
Cya1
-
Synechocystis sp. PCC 6803