Literature summary for 4.6.1.1 extracted from

Tesmer, J.J.G.; Sunahara, R.K.; Johnson, R.A.; Gosselin, G.; Gilman, A.G.; Sprang, S.R.
Two-metal-ion catalysis in adenylyl cyclase (1999), Science, 285, 756-760.

Search for more literature for 4.6.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression of soluble AC in Escherichia coli	Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of native soluble AC and of soluble AC in complex with the competitiv substrate analogs beta-L-2',3'-dideoxyadenosine 5'-triphiosphate and adenosine 5'-(alpha-thio)-triphosphate at 2.8-3.0 A	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
D396A	almost no activity	Rattus norvegicus
D396A/D440A	no activity	Rattus norvegicus
D396A/D440N	no activity	Rattus norvegicus
D396N	almost no activity	Rattus norvegicus
D396N/D440A	no activity	Rattus norvegicus
D396N/D440N	no activity	Rattus norvegicus
D440A	almost no activity	Rattus norvegicus
D440N	almost no activity	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.094	-	ATP	after activation of soluble AC with the stimulatory G protein alpha protein and GTP-gamma-S in the presence of Mn2+	Rattus norvegicus
0.56	-	ATP	after activation of soluble AC with the stimulatory G protein alpha protein and GTP-gamma-S in the presence of Mg2+	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Rattus norvegicus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	activates	Rattus norvegicus
Mn2+	activates	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP	Rattus norvegicus	-	3',5'-cyclic AMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant soluble AC	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP	-	Rattus norvegicus	3',5'-cyclic AMP + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
adenylyl cyclase	-	Rattus norvegicus
soluble AC	-	Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
8.8	-	ATP	after activation of soluble AC with the stimulatory G protein alpha protein and GTP-gamma-S in the presence of Mn2+	Rattus norvegicus
15.5	-	ATP	after activation of soluble AC with the stimulatory G protein alpha protein and GTP-gamma-S in the presence of Mg2+	Rattus norvegicus

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Release 2023.1 (January 2023)