Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
R18A | 88% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
R18A/R19G | 67% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
R19G | 78% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
R27A | 78% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
R31G | 118% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
R43A | 61% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
R43A/R44G | 1.3% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
R43K | 77% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
R43K/R44K | 39% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
R44G | 41% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
R44K | 116% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
R46A | 85% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
R46K | 135% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
R4G | 77% of wild-type activity, in the presence of 2 mM Mn2+ | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
ATP | recombinant adenylyl cclase, in the presence of Mn2+ | Mycobacterium tuberculosis | |
3.5 | - |
ATP | recombinant adenylyl cclase, in the presence of Mg2+ | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | six putative helical transmembrane domains | Mycobacterium tuberculosis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 1 mM, 47fold activation | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | Mycobacterium tuberculosis | - |
3',5'-cyclic AMP + diphosphate | - |
? | |
ATP | Mycobacterium tuberculosis H37Rv | - |
3',5'-cyclic AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WQ35 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WQ35 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant adenylyl cyclase | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.48 | - |
in the presence of physiological ATP concentrations and 1 mM Mn2+ | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | - |
Mycobacterium tuberculosis | 3',5'-cyclic AMP + diphosphate | - |
? | |
ATP | - |
Mycobacterium tuberculosis H37Rv | 3',5'-cyclic AMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
adenylyl cyclase | - |
Mycobacterium tuberculosis |
Rv1625c | - |
Mycobacterium tuberculosis |