KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane | pH 7.0, 37°C | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | a metalloprotein containing Zn2+, Mg2+, and Ca2+ ions | Pseudomonas putida | |
Mg2+ | a metalloprotein containing Zn2+, Mg2+, and Ca2+ ions | Pseudomonas putida | |
Zn2+ | a metalloprotein containing Zn2+, Mg2+, and Ca2+ ions | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
about, native PAGE | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane | Pseudomonas putida | - |
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride | - |
? | |
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane | Pseudomonas putida T5 | - |
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida T5 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | a glycoprotein with mannose forming the backbone | Pseudomonas putida |
Purification (Comment) | Organism |
---|---|
native enzyme from strain T5 to homogeneity | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane | - |
Pseudomonas putida | 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride | - |
? | |
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane | - |
Pseudomonas putida T5 | 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Pseudomonas putida |
More | mass spectrometric sequence canalysis | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
DDT-dehydrohalogenase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
purified native enzyme, pH 7.0, stable for 4-5 h | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.113 | - |
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane | pH 7.0, 37°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
purified native enzyme, 37°C, stable for 4-5 h | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
additional information | serine and tryptophan appear to be involved at the active site | Pseudomonas putida |
physiological function | the enzyme is involved in the catalytic degradation of 4,4'-DDT by eliminating either HCl or Cl- to form DDD/DDE | Pseudomonas putida |