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Literature summary for 4.5.1.1 extracted from

  • Rangachary, L.; Rajagopalan, R.P.; Singh, T.M.; Krishnan, M.H.
    Purification and characterization of DDT-dehydrohalogenase from Pseudomonas putida T5 (2012), Prep. Biochem. Biotechnol., 42, 60-76.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane pH 7.0, 37°C Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ a metalloprotein containing Zn2+, Mg2+, and Ca2+ ions Pseudomonas putida
Mg2+ a metalloprotein containing Zn2+, Mg2+, and Ca2+ ions Pseudomonas putida
Zn2+ a metalloprotein containing Zn2+, Mg2+, and Ca2+ ions Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
about, native PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane Pseudomonas putida
-
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane Pseudomonas putida T5
-
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida T5
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein a glycoprotein with mannose forming the backbone Pseudomonas putida

Purification (Commentary)

Purification (Comment) Organism
native enzyme from strain T5 to homogeneity Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
-
Pseudomonas putida 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
-
Pseudomonas putida T5 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
-
?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE Pseudomonas putida
More mass spectrometric sequence canalysis Pseudomonas putida

Synonyms

Synonyms Comment Organism
DDT-dehydrohalogenase
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
purified native enzyme, pH 7.0, stable for 4-5 h Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.113
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane pH 7.0, 37°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
purified native enzyme, 37°C, stable for 4-5 h Pseudomonas putida

General Information

General Information Comment Organism
additional information serine and tryptophan appear to be involved at the active site Pseudomonas putida
physiological function the enzyme is involved in the catalytic degradation of 4,4'-DDT by eliminating either HCl or Cl- to form DDD/DDE Pseudomonas putida