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Literature summary for 4.4.1.4 extracted from

  • Li, J.W.; Xia, L.; Su, Y.; Liu, H.; Xia, X.; Lu, Q.; Yang, C.; Reheman, K.
    Molecular imprint of enzyme active site by camel nanobodies: rapid and efficient approach to produce abzymes with alliinase activity (2012), J. Biol. Chem., 287, 13713-13721.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information production of antibodies with alliinase activities by exploiting the molecular mimicry of Camelus bactrianus nanobodies: enrichment of variable domain of the heavy chain of a heavy-chain antibodies specific against the alliinase via phage display, recombinant expression of the selected His-tagged antibodies in Escherichia coli strain BL21(DE3), and purification by nickel affinity chromatography Allium sativum

Inhibitors

Inhibitors Comment Organism Structure
additional information generation and selection, as well as evaluation of variable domain of the heavy chain of a heavy-chain antibodies specific against the alliinase with inhibitory potency against alliinase, inhibitory nanobody VHHA4 that recognizes the active site. Inhibition of B16 F10 cell proliferation by antibody VHH C10 in the presence of alliin, inhibition kinetics, overview Allium sativum
Penicillamine competitive inhibitor Allium sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.32
-
4-mercaptopyridine catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C Allium sativum
1.418
-
4-mercaptopyridine native enzyme, pH 7.0, 35°C Allium sativum
1.493
-
(+/-)-alliin native enzyme, pH 7.0, 35°C Allium sativum
1.521
-
(+/-)-alliin catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C Allium sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+/-)-alliin Allium sativum
-
allicin + pyruvic acid + NH3
-
?
additional information Allium sativum cell viability of B16 F10 (B16) cells treated with catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibodies, VHHs, is reduced ?
-
?

Organism

Organism UniProt Comment Textmining
Allium sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by gel filtration and lectin concanavalin A affinity chromatography Allium sativum

Source Tissue

Source Tissue Comment Organism Textmining
clove
-
Allium sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
59 1003 purified catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibodies, pH 7.0, 35°C, substrate (+/-)-alliin Allium sativum
2989
-
purifed native enzyme, pH 7.0, 35°C, substrate (+/-)-alliin Allium sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+/-)-alliin
-
Allium sativum allicin + pyruvic acid + NH3
-
?
(+/-)-alliin carbon-sulfur (C-S) bond lyase activity of alliinase and catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibodies, VHHs, overview Allium sativum allicin + pyruvic acid + NH3
-
?
4-mercaptopyridine
-
Allium sativum ?
-
?
additional information cell viability of B16 F10 (B16) cells treated with catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibodies, VHHs, is reduced Allium sativum ?
-
?

Synonyms

Synonyms Comment Organism
alliinase
-
Allium sativum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Allium sativum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00185
-
4-mercaptopyridine catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C Allium sativum
0.0028
-
4-mercaptopyridine native enzyme, pH 7.0, 35°C Allium sativum
0.008
-
(+/-)-alliin catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C Allium sativum
0.015
-
(+/-)-alliin native enzyme, pH 7.0, 35°C Allium sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Allium sativum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Allium sativum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.084
-
4-mercaptopyridine catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C Allium sativum
0.11
-
4-mercaptopyridine native enzyme, pH 7.0, 35°C Allium sativum
0.331
-
(+/-)-alliin catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C Allium sativum
0.595
-
(+/-)-alliin native enzyme, pH 7.0, 35°C Allium sativum