Protein Variants | Comment | Organism |
---|---|---|
additional information | production of antibodies with alliinase activities by exploiting the molecular mimicry of Camelus bactrianus nanobodies: enrichment of variable domain of the heavy chain of a heavy-chain antibodies specific against the alliinase via phage display, recombinant expression of the selected His-tagged antibodies in Escherichia coli strain BL21(DE3), and purification by nickel affinity chromatography | Allium sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | generation and selection, as well as evaluation of variable domain of the heavy chain of a heavy-chain antibodies specific against the alliinase with inhibitory potency against alliinase, inhibitory nanobody VHHA4 that recognizes the active site. Inhibition of B16 F10 cell proliferation by antibody VHH C10 in the presence of alliin, inhibition kinetics, overview | Allium sativum | |
Penicillamine | competitive inhibitor | Allium sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.32 | - |
4-mercaptopyridine | catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C | Allium sativum | |
1.418 | - |
4-mercaptopyridine | native enzyme, pH 7.0, 35°C | Allium sativum | |
1.493 | - |
(+/-)-alliin | native enzyme, pH 7.0, 35°C | Allium sativum | |
1.521 | - |
(+/-)-alliin | catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C | Allium sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+/-)-alliin | Allium sativum | - |
allicin + pyruvic acid + NH3 | - |
? | |
additional information | Allium sativum | cell viability of B16 F10 (B16) cells treated with catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibodies, VHHs, is reduced | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Allium sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by gel filtration and lectin concanavalin A affinity chromatography | Allium sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
clove | - |
Allium sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
59 | 1003 | purified catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibodies, pH 7.0, 35°C, substrate (+/-)-alliin | Allium sativum |
2989 | - |
purifed native enzyme, pH 7.0, 35°C, substrate (+/-)-alliin | Allium sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+/-)-alliin | - |
Allium sativum | allicin + pyruvic acid + NH3 | - |
? | |
(+/-)-alliin | carbon-sulfur (C-S) bond lyase activity of alliinase and catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibodies, VHHs, overview | Allium sativum | allicin + pyruvic acid + NH3 | - |
? | |
4-mercaptopyridine | - |
Allium sativum | ? | - |
? | |
additional information | cell viability of B16 F10 (B16) cells treated with catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibodies, VHHs, is reduced | Allium sativum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alliinase | - |
Allium sativum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Allium sativum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00185 | - |
4-mercaptopyridine | catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C | Allium sativum | |
0.0028 | - |
4-mercaptopyridine | native enzyme, pH 7.0, 35°C | Allium sativum | |
0.008 | - |
(+/-)-alliin | catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C | Allium sativum | |
0.015 | - |
(+/-)-alliin | native enzyme, pH 7.0, 35°C | Allium sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Allium sativum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Allium sativum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.084 | - |
4-mercaptopyridine | catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C | Allium sativum | |
0.11 | - |
4-mercaptopyridine | native enzyme, pH 7.0, 35°C | Allium sativum | |
0.331 | - |
(+/-)-alliin | catalytic alliinase-specific variable domain of the heavy chain of a heavy-chain antibody VHH C-10, pH 7.0, 35°C | Allium sativum | |
0.595 | - |
(+/-)-alliin | native enzyme, pH 7.0, 35°C | Allium sativum |