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Literature summary for 4.4.1.4 extracted from

  • Won, T.; Mazelis, M.
    The C-S lyases of higher plants. Purification and characterization of homogeneous alliin lyase of leek (Allium porrum) (1989), Physiol. Plant., 77, 87-92.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Aminooxyacetate
-
Allium ampeloprasum
hydroxylamine
-
Allium ampeloprasum
NaCN
-
Allium ampeloprasum
Trinitrobenzenesulfonic acid 2.5 mM, 50% inhibition Allium ampeloprasum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10
-
S-allyl-L-Cys sulfoxide
-
Allium ampeloprasum
13
-
S-ethyl-L-Cys sulfoxide
-
Allium ampeloprasum
55
-
S-methyl-L-Cys sulfoxide
-
Allium ampeloprasum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
x * 48000, SDS-PAGE Allium ampeloprasum
386000
-
2 stable enzyme forms detected: MW 386000 Da and 580000 Da, gel filtration Allium ampeloprasum
580000
-
2 stable enzyme forms detected: MW 386000 Da and 580000 Da, gel filtration Allium ampeloprasum

Organism

Organism UniProt Comment Textmining
Allium ampeloprasum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Allium ampeloprasum
side-chain modification major sugars are mannose, glucose and galactose Allium ampeloprasum

Purification (Commentary)

Purification (Comment) Organism
-
Allium ampeloprasum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-ethyl-L-Cys sulfoxide + H2O
-
Allium ampeloprasum ethyl ethanethiosulfinate + 2 pyruvate + 2 NH3
-
?
S-allyl-L-cysteine sulfoxide + H2O
-
Allium ampeloprasum 2-propene-thioic acid + pyruvate + NH4+ + H+
-
?
S-methyl-L-Cys sulfoxide 40% of the activity with S-ethyl-L-Cys sulfoxide Allium ampeloprasum ?
-
?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Allium ampeloprasum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Allium ampeloprasum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5
-
Trinitrobenzenesulfonic acid
-
Allium ampeloprasum