Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | probably by preventing autooxidation of substrate | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aminooxyacetic acid | - |
Spinacia oleracea | |
Co2+ | - |
Spinacia oleracea | |
hydroxylamine | - |
Spinacia oleracea | |
Ni2+ | - |
Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-cysteine + H2O | Spinacia oleracea | plant sulfur metabolism | sulfide + NH3 + pyruvate | additional product suggested | ? | |
D-cysteine + H2O | Spinacia oleracea | plant sulfur metabolism | sulfide + NH3 + pyruvate | more sulfide than pyruvate and ammonium formed | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
purified enzyme, 37°C, pH 9.0 | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-cysteine + H2O | very specific, no activity with L-cysteine, mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester and dithioerythritol | Spinacia oleracea | sulfide + NH3 + pyruvate | additional product suggested | ? | |
D-cysteine + H2O | very specific, no activity with L-cysteine, mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester and dithioerythritol | Spinacia oleracea | sulfide + NH3 + pyruvate | more sulfide than pyruvate and ammonium formed | ? | |
D-cysteine + H2O | plant sulfur metabolism | Spinacia oleracea | sulfide + NH3 + pyruvate | additional product suggested | ? | |
D-cysteine + H2O | plant sulfur metabolism | Spinacia oleracea | sulfide + NH3 + pyruvate | more sulfide than pyruvate and ammonium formed | ? | |
additional information | L-cysteine is not utilized | Spinacia oleracea | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Spinacia oleracea |