Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | - |
Escherichia coli | |
D-penicillamine | - |
Escherichia coli | |
HgCl2 | - |
Escherichia coli | |
hydroxylamine | - |
Escherichia coli | |
L-cysteine | mixed type inhibition | Escherichia coli | |
L-Penicillamine | - |
Escherichia coli | |
additional information | no inhibition with EDTA and substrates | Escherichia coli | |
p-chloromercuribenzoate | - |
Escherichia coli | |
phenylhydrazine | - |
Escherichia coli | |
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
DL-selenocystine | 30°C, pH 8.0, based on the concentration of the D-isomer, Escherichia coli | Escherichia coli | |
0.11 | - |
DL-lanthionine | 30°C, pH 8.0, based on the concentration of the D-isomer, Escherichia coli | Escherichia coli | |
0.15 | - |
D-cysteine | 30°C, pH 8.0 | Escherichia coli | |
0.22 | - |
S-phenyl-D-cysteine | 30°C, pH 8.0 | Escherichia coli | |
0.23 | - |
S-n-propyl-D-cysteine | 30°C, pH 8.0 | Escherichia coli | |
0.24 | - |
S-n-butyl-D-cysteine | 30°C, pH 8.0 | Escherichia coli | |
0.27 | - |
D-Cystine | 30°C, pH 8.0 | Escherichia coli | |
0.28 | - |
S-benzyl-D-cysteine | 30°C, pH 8.0 | Escherichia coli | |
0.29 | - |
D-allocystathionine | 30°C, pH 8.0 | Escherichia coli | |
0.42 | - |
S-ethyl-D-cysteine | 30°C, pH 8.0 | Escherichia coli | |
0.5 | - |
S-methyl-D-cysteine | 30°C, pH 8.0 | Escherichia coli | |
0.83 | - |
S-carboxymethyl-D-cysteine | 30°C, pH 8.0 | Escherichia coli | |
0.91 | - |
3-chloro-D-alanine | 30°C, pH 8.0, alpha,beta-elimination | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
SDS-PAGE | Escherichia coli |
67000 | - |
sedimentation equilibrium centrifugation | Escherichia coli |
70000 | - |
gel filtration | Escherichia coli |
74000 | - |
HPLC gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-cysteine + H2O | Escherichia coli | - |
sulfide + NH3 + pyruvate | - |
? | |
D-cysteine + H2O | Enterobacter cloacae | - |
sulfide + NH3 + pyruvate | - |
? | |
D-cysteine + H2O | Klebsiella pneumoniae | - |
sulfide + NH3 + pyruvate | - |
? | |
D-cysteine + H2O | Citrobacter freundii | - |
sulfide + NH3 + pyruvate | - |
? | |
D-cysteine + H2O | Escherichia coli W3110 / ATCC 27325 | - |
sulfide + NH3 + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | - |
- |
- |
Enterobacter cloacae | - |
- |
- |
Escherichia coli | - |
deltatrpED102/F'deltatrpED102 | - |
Escherichia coli W3110 / ATCC 27325 | - |
deltatrpED102/F'deltatrpED102 | - |
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13 | - |
purified enzyme, 30°C, pH 8.0, with D-cysteine as substrate | Escherichia coli |
56.3 | - |
purified enzyme, 30°C, pH 8.0, with 3-chloro-D-alanine as substrate | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 10 mM phosphate buffer, pH 7, 0.01 mM pyridoxal phosphate, 0.1 mM DTT, 0.1 mM EDTA 50% glycerol, at least 2 months, no loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chloro-D-alanine + H2O | alpha,beta-elimination, more effective substrate than D-cysteine | Escherichia coli | pyruvate + NH3 + Cl- | - |
? | |
3-chloro-D-alanine + H2O | alpha,beta-elimination, more effective substrate than D-cysteine | Escherichia coli W3110 / ATCC 27325 | pyruvate + NH3 + Cl- | - |
? | |
3-chloro-D-alanine + thioglycolic acid | i.e. mercaptoacetic acid, beta-replacement reaction | Escherichia coli | S-carboxymethyl-D-cysteine | product is further metabolized to form pyruvate | ? | |
3-chloro-D-alanine + thioglycolic acid | i.e. mercaptoacetic acid, beta-replacement reaction | Escherichia coli W3110 / ATCC 27325 | S-carboxymethyl-D-cysteine | product is further metabolized to form pyruvate | ? | |
D-allocystathionine + H2O | - |
Escherichia coli | ? | - |
? | |
D-cysteine + H2O | - |
Escherichia coli | sulfide + NH3 + pyruvate | - |
? | |
D-cysteine + H2O | - |
Enterobacter cloacae | sulfide + NH3 + pyruvate | - |
? | |
D-cysteine + H2O | - |
Klebsiella pneumoniae | sulfide + NH3 + pyruvate | - |
? | |
D-cysteine + H2O | - |
Citrobacter freundii | sulfide + NH3 + pyruvate | - |
? | |
D-cysteine + H2O | alpha,beta-elimination | Escherichia coli | sulfide + NH3 + pyruvate | - |
? | |
D-cysteine + H2O | - |
Escherichia coli W3110 / ATCC 27325 | sulfide + NH3 + pyruvate | - |
? | |
D-cysteine + H2O | alpha,beta-elimination | Escherichia coli W3110 / ATCC 27325 | sulfide + NH3 + pyruvate | - |
? | |
D-cystine + H2O | - |
Escherichia coli | ? | - |
? | |
DL-lanthionine + H2O | - |
Escherichia coli | ? | - |
? | |
DL-selenocysteine + H2O | very poor substrate | Escherichia coli | selenide + L-alanine | - |
? | |
DL-selenocystine + H2O | - |
Escherichia coli | ? | - |
? | |
additional information | L-cysteine is not utilized | Escherichia coli | ? | - |
? | |
additional information | L-cysteine is not utilized | Enterobacter cloacae | ? | - |
? | |
additional information | L-cysteine is not utilized | Klebsiella pneumoniae | ? | - |
? | |
additional information | L-cysteine is not utilized | Citrobacter freundii | ? | - |
? | |
additional information | L-cysteine is not utilized | Escherichia coli W3110 / ATCC 27325 | ? | - |
? | |
O-methyl-DL-serine + H2O | poor substrate | Escherichia coli | ? | - |
? | |
S-benzyl-D-cysteine + H2O | - |
Escherichia coli | benzyl hydrosulfide + pyruvate + NH3 | - |
? | |
S-carboxymethyl-D-cysteine + H2O | - |
Escherichia coli | mercaptoacetic acid + pyruvate + NH3 | - |
? | |
S-ethyl-D-cysteine + H2O | - |
Escherichia coli | ethanethiol + pyruvate + NH3 | - |
? | |
S-methyl-D-cysteine + H2O | - |
Escherichia coli | methanethiol + pyruvate + NH3 | - |
? | |
S-n-butyl-D-cysteine + H2O | - |
Escherichia coli | butane-1-thiol + pyruvate + NH3 | - |
? | |
S-n-propyl-D-cysteine + H2O | - |
Escherichia coli | propane-1-thiol + pyruvate + NH3 | - |
? | |
S-phenyl-D-cysteine + H2O | - |
Escherichia coli | benzenthiol + pyruvate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36500, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
no loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0 | Escherichia coli |
65 | - |
11% loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0 | Escherichia coli |
70 | - |
24% loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0 | Escherichia coli |
75 | - |
40% loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0 | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
alpha,beta-elimination | Escherichia coli |
9.5 | - |
beta-replacement | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 2 mol per mol enzyme | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
L-cysteine | - |
Escherichia coli |