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Literature summary for 4.4.1.15 extracted from

  • Nagasawa, T.; Ishii, T.; Kumagai, H.; Yamada, H.
    D-Cysteine desulfhydrase of Escherichia coli. Purification and characterization (1985), Eur. J. Biochem., 153, 541-551.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
Escherichia coli
D-penicillamine
-
Escherichia coli
HgCl2
-
Escherichia coli
hydroxylamine
-
Escherichia coli
L-cysteine mixed type inhibition Escherichia coli
L-Penicillamine
-
Escherichia coli
additional information no inhibition with EDTA and substrates Escherichia coli
p-chloromercuribenzoate
-
Escherichia coli
phenylhydrazine
-
Escherichia coli
Zn2+
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
DL-selenocystine 30°C, pH 8.0, based on the concentration of the D-isomer, Escherichia coli Escherichia coli
0.11
-
DL-lanthionine 30°C, pH 8.0, based on the concentration of the D-isomer, Escherichia coli Escherichia coli
0.15
-
D-cysteine 30°C, pH 8.0 Escherichia coli
0.22
-
S-phenyl-D-cysteine 30°C, pH 8.0 Escherichia coli
0.23
-
S-n-propyl-D-cysteine 30°C, pH 8.0 Escherichia coli
0.24
-
S-n-butyl-D-cysteine 30°C, pH 8.0 Escherichia coli
0.27
-
D-Cystine 30°C, pH 8.0 Escherichia coli
0.28
-
S-benzyl-D-cysteine 30°C, pH 8.0 Escherichia coli
0.29
-
D-allocystathionine 30°C, pH 8.0 Escherichia coli
0.42
-
S-ethyl-D-cysteine 30°C, pH 8.0 Escherichia coli
0.5
-
S-methyl-D-cysteine 30°C, pH 8.0 Escherichia coli
0.83
-
S-carboxymethyl-D-cysteine 30°C, pH 8.0 Escherichia coli
0.91
-
3-chloro-D-alanine 30°C, pH 8.0, alpha,beta-elimination Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36500
-
SDS-PAGE Escherichia coli
67000
-
sedimentation equilibrium centrifugation Escherichia coli
70000
-
gel filtration Escherichia coli
74000
-
HPLC gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-cysteine + H2O Escherichia coli
-
sulfide + NH3 + pyruvate
-
?
D-cysteine + H2O Enterobacter cloacae
-
sulfide + NH3 + pyruvate
-
?
D-cysteine + H2O Klebsiella pneumoniae
-
sulfide + NH3 + pyruvate
-
?
D-cysteine + H2O Citrobacter freundii
-
sulfide + NH3 + pyruvate
-
?
D-cysteine + H2O Escherichia coli W3110 / ATCC 27325
-
sulfide + NH3 + pyruvate
-
?

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
-
-
Enterobacter cloacae
-
-
-
Escherichia coli
-
deltatrpED102/F'deltatrpED102
-
Escherichia coli W3110 / ATCC 27325
-
deltatrpED102/F'deltatrpED102
-
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13
-
purified enzyme, 30°C, pH 8.0, with D-cysteine as substrate Escherichia coli
56.3
-
purified enzyme, 30°C, pH 8.0, with 3-chloro-D-alanine as substrate Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 10 mM phosphate buffer, pH 7, 0.01 mM pyridoxal phosphate, 0.1 mM DTT, 0.1 mM EDTA 50% glycerol, at least 2 months, no loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chloro-D-alanine + H2O alpha,beta-elimination, more effective substrate than D-cysteine Escherichia coli pyruvate + NH3 + Cl-
-
?
3-chloro-D-alanine + H2O alpha,beta-elimination, more effective substrate than D-cysteine Escherichia coli W3110 / ATCC 27325 pyruvate + NH3 + Cl-
-
?
3-chloro-D-alanine + thioglycolic acid i.e. mercaptoacetic acid, beta-replacement reaction Escherichia coli S-carboxymethyl-D-cysteine product is further metabolized to form pyruvate ?
3-chloro-D-alanine + thioglycolic acid i.e. mercaptoacetic acid, beta-replacement reaction Escherichia coli W3110 / ATCC 27325 S-carboxymethyl-D-cysteine product is further metabolized to form pyruvate ?
D-allocystathionine + H2O
-
Escherichia coli ?
-
?
D-cysteine + H2O
-
Escherichia coli sulfide + NH3 + pyruvate
-
?
D-cysteine + H2O
-
Enterobacter cloacae sulfide + NH3 + pyruvate
-
?
D-cysteine + H2O
-
Klebsiella pneumoniae sulfide + NH3 + pyruvate
-
?
D-cysteine + H2O
-
Citrobacter freundii sulfide + NH3 + pyruvate
-
?
D-cysteine + H2O alpha,beta-elimination Escherichia coli sulfide + NH3 + pyruvate
-
?
D-cysteine + H2O
-
Escherichia coli W3110 / ATCC 27325 sulfide + NH3 + pyruvate
-
?
D-cysteine + H2O alpha,beta-elimination Escherichia coli W3110 / ATCC 27325 sulfide + NH3 + pyruvate
-
?
D-cystine + H2O
-
Escherichia coli ?
-
?
DL-lanthionine + H2O
-
Escherichia coli ?
-
?
DL-selenocysteine + H2O very poor substrate Escherichia coli selenide + L-alanine
-
?
DL-selenocystine + H2O
-
Escherichia coli ?
-
?
additional information L-cysteine is not utilized Escherichia coli ?
-
?
additional information L-cysteine is not utilized Enterobacter cloacae ?
-
?
additional information L-cysteine is not utilized Klebsiella pneumoniae ?
-
?
additional information L-cysteine is not utilized Citrobacter freundii ?
-
?
additional information L-cysteine is not utilized Escherichia coli W3110 / ATCC 27325 ?
-
?
O-methyl-DL-serine + H2O poor substrate Escherichia coli ?
-
?
S-benzyl-D-cysteine + H2O
-
Escherichia coli benzyl hydrosulfide + pyruvate + NH3
-
?
S-carboxymethyl-D-cysteine + H2O
-
Escherichia coli mercaptoacetic acid + pyruvate + NH3
-
?
S-ethyl-D-cysteine + H2O
-
Escherichia coli ethanethiol + pyruvate + NH3
-
?
S-methyl-D-cysteine + H2O
-
Escherichia coli methanethiol + pyruvate + NH3
-
?
S-n-butyl-D-cysteine + H2O
-
Escherichia coli butane-1-thiol + pyruvate + NH3
-
?
S-n-propyl-D-cysteine + H2O
-
Escherichia coli propane-1-thiol + pyruvate + NH3
-
?
S-phenyl-D-cysteine + H2O
-
Escherichia coli benzenthiol + pyruvate + NH3
-
?

Subunits

Subunits Comment Organism
dimer 2 * 36500, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
no loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0 Escherichia coli
65
-
11% loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0 Escherichia coli
70
-
24% loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0 Escherichia coli
75
-
40% loss of activity within 30 min incubation, 0.1 M phosphate buffer, pH 8.0 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
alpha,beta-elimination Escherichia coli
9.5
-
beta-replacement Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 2 mol per mol enzyme Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.53
-
L-cysteine
-
Escherichia coli