Crystallization (Comment) | Organism |
---|---|
the crystal structure of STR1 in complex with strictosidine is solved at a resolution of 3.0 A | Rauvolfia serpentina |
Protein Variants | Comment | Organism |
---|---|---|
V208A | Val208 plays a critical role in substrate recognition at the indole moiety of strictosidine | Rauvolfia serpentina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
tryptamine | wild-type enzyme | Rauvolfia serpentina | |
0.136 | - |
6-fluoro-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
0.219 | - |
tryptamine | mutant V208A | Rauvolfia serpentina | |
0.259 | - |
5-fluoro-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
0.281 | - |
5-methyl-tryptamine | mutant V208A | Rauvolfia serpentina | |
0.307 | - |
6-methoxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
0.356 | - |
6-fluoro-tryptamine | mutant V208A | Rauvolfia serpentina | |
0.393 | - |
6-methyl-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
0.762 | - |
6-methyl-tryptamine | mutant V208A | Rauvolfia serpentina | |
0.844 | - |
5-hydroxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
0.962 | - |
6-methoxy-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
1.302 | - |
5-fluoro-tryptamine | mutant V208A | Rauvolfia serpentina | |
2.255 | - |
5-hydroxy-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
3.592 | - |
5-methoxy-tryptamine | mutant V208A | Rauvolfia serpentina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-alpha(S)-strictosidine + H2O | Rauvolfia serpentina | - |
tryptamine + secologanin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rauvolfia serpentina | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-alpha(S)-strictosidine + H2O | - |
Rauvolfia serpentina | tryptamine + secologanin | - |
? | |
5-fluoro-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? | |
5-hydroxy-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? | |
5-methoxy-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? | |
5-methyl-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? | |
6-fluoro-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? | |
6-methoxy-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? | |
6-methyl-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
STR1 | - |
Rauvolfia serpentina |
strictosidine synthase | - |
Rauvolfia serpentina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
5-hydroxy-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
0.03 | 0.55 | 5-methyl-tryptamine | mutant V208A | Rauvolfia serpentina | |
0.057 | 0.65 | tryptamine | wild-type enzyme | Rauvolfia serpentina | |
0.057 | 0.65 | 6-methyl-tryptamine | mutant V208A | Rauvolfia serpentina | |
0.21 | - |
5-methoxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
0.52 | - |
6-fluoro-tryptamine | mutant V208A | Rauvolfia serpentina | |
0.64 | - |
tryptamine | mutant V208A | Rauvolfia serpentina | |
0.97 | - |
6-methoxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
0.99 | - |
5-hydroxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
2.32 | - |
6-methyl-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
3.4 | - |
5-fluoro-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
3.8 | - |
5-fluoro-tryptamine | mutant V208A | Rauvolfia serpentina | |
5.32 | - |
6-methoxy-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
6.56 | - |
5-methyl-tryptamine | mutant V208A | Rauvolfia serpentina | |
10.65 | - |
tryptamine | wild-type enzyme | Rauvolfia serpentina | |
10.95 | - |
6-methyl-tryptamine | mutant V208A | Rauvolfia serpentina | |
13.63 | - |
6-fluoro-tryptamine | mutant V208A | Rauvolfia serpentina | |
16.66 | - |
6-methoxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
16.9 | - |
6-fluoro-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
18.12 | - |
5-hydroxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
21.24 | - |
5-fluoro-tryptamine | mutant V208A | Rauvolfia serpentina | |
23.37 | - |
6-fluoro-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
37.46 | - |
5-fluoro-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
54.09 | - |
tryptamine | mutant V208A | Rauvolfia serpentina | |
79.66 | - |
5-methoxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
562.1 | - |
5-hydroxy-tryptamine | wild-type enzyme | Rauvolfia serpentina |