Application | Comment | Organism |
---|---|---|
biotechnology | redesigning the substrate specificity of strictosidine synthase is an important step toward re-engineering the TIA pathway to produce products with novel or improved biological properties | Catharanthus roseus |
Cloned (Comment) | Organism |
---|---|
for expression in Escherichia coli BL21DE3 cells | Catharanthus roseus |
Protein Variants | Comment | Organism |
---|---|---|
D177A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
E277G | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
F232T | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
F314A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
F330L | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
H283A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
H313N | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
L329G | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
Q282A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
S275G | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
V182G | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
W153F | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
Y109H | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
Y155A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-alpha(S)-strictosidine + H2O | Catharanthus roseus | - |
tryptamine + secologanin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by one-step affinity purification using a amylose column | Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-alpha(S)-strictosidine + H2O | - |
Catharanthus roseus | tryptamine + secologanin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
str | - |
Catharanthus roseus |
strictosidine synthase | - |
Catharanthus roseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
HPLC activity assay | Catharanthus roseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
HPLC activity assay | Catharanthus roseus |