General Stability | Organism |
---|---|
high loss of activity on repeated freezing and thawing | Catharanthus roseus |
stable on repeated freezing and thawing | Catharanthus roseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition with secologanin | Catharanthus roseus | |
p-chloromercuribenzoate | - |
Amsonia orientalis | |
p-chloromercuribenzoate | - |
Amsonia salicifolia | |
p-chloromercuribenzoate | - |
Catharanthus roseus | |
p-chloromercuribenzoate | - |
Ochrosia elliptica | |
p-chloromercuribenzoate | - |
Rauvolfia vomitoria | |
p-chloromercuribenzoate | - |
Stemmadenia tomentosa var. palmeri | |
p-chloromercuribenzoate | - |
Vinca major | |
p-chloromercuribenzoate | - |
Voacanga africana | |
tryptamine | inhibitory above 1.2 mM | Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
tryptamine | pH 7.0, 35°C | Catharanthus roseus | |
3.4 | - |
secologanin | pH 7.0, 35°C | Catharanthus roseus | |
3.5 | - |
dihydrosecologanin | pH 7.0, 35°C | Catharanthus roseus | |
8 | - |
3'-O-methylsecologanin | pH 7.0, 35°C | Catharanthus roseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Catharanthus roseus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
gel filtration | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tryptamine + secologanin | Catharanthus roseus | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Amsonia salicifolia | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Catharanthus pusillus | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Catharanthus trichophyllus | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Rauvolfia vomitoria | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Amsonia orientalis | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Vinca major | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Rauvolfia verticillata | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Vinca minor | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Amsonia tabernaemontana | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Catharanthus longifolius | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Ervatamia divaricatum | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Ochrosia elliptica | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Stemmadenia tomentosa var. palmeri | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | Voacanga africana | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amsonia orientalis | - |
- |
- |
Amsonia salicifolia | - |
- |
- |
Amsonia tabernaemontana | - |
- |
- |
Catharanthus longifolius | - |
- |
- |
Catharanthus pusillus | - |
- |
- |
Catharanthus roseus | - |
i.e. Vinca rosea | - |
Catharanthus trichophyllus | - |
- |
- |
Ervatamia divaricatum | - |
- |
- |
Ochrosia elliptica | - |
- |
- |
Rauvolfia verticillata | - |
- |
- |
Rauvolfia vomitoria | - |
- |
- |
Stemmadenia tomentosa var. palmeri | - |
- |
- |
Vinca major | - |
- |
- |
Vinca minor | - |
- |
- |
Voacanga africana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Catharanthus roseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Catharanthus roseus | - |
cell culture | - |
Amsonia salicifolia | - |
cell culture | - |
Rauvolfia vomitoria | - |
cell culture | - |
Amsonia orientalis | - |
cell culture | - |
Vinca major | - |
cell culture | - |
Ochrosia elliptica | - |
cell culture | - |
Stemmadenia tomentosa var. palmeri | - |
cell culture | - |
Voacanga africana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.07 | - |
purified enzyme, pH 7.0, 35°C | Catharanthus roseus |
Storage Stability | Organism |
---|---|
-20°, 1 year, no loss of activity | Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorotryptamine + secologanin | 6% of the activity with trypamine | Catharanthus roseus | ? | - |
? | |
5-hydroxytryptamine + secologanin | 4% of the activity with trypamine | Catharanthus roseus | ? | - |
? | |
6-hydroxytryptamine + secologanin | 9% of the activity with trypamine | Catharanthus roseus | ? | - |
? | |
7-fluorotryptamine + secologanin | 8% of the activity with trypamine | Catharanthus roseus | ? | - |
? | |
7-methyltryptamine + secologanin | 15% of the activity with trypamine | Catharanthus roseus | ? | - |
? | |
tryptamine + 2'-O-methylsecologanin | - |
Catharanthus roseus | ? | - |
? | |
tryptamine + 3'-O-methylsecologanin | - |
Catharanthus roseus | ? | - |
? | |
tryptamine + dihydrosecologanin | - |
Catharanthus roseus | ? | - |
? | |
tryptamine + secologanin | - |
Amsonia salicifolia | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | - |
Catharanthus pusillus | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | - |
Catharanthus trichophyllus | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | - |
Rauvolfia vomitoria | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | - |
Amsonia orientalis | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | - |
Vinca major | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | - |
Rauvolfia verticillata | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | - |
Vinca minor | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | - |
Amsonia tabernaemontana | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | - |
Catharanthus longifolius | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | - |
Ervatamia divaricatum | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | - |
Ochrosia elliptica | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | - |
Stemmadenia tomentosa var. palmeri | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | - |
Voacanga africana | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | highly specific | Catharanthus roseus | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Catharanthus roseus | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Amsonia salicifolia | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Catharanthus pusillus | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Catharanthus trichophyllus | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Rauvolfia vomitoria | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Amsonia orientalis | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Vinca major | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Rauvolfia verticillata | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Vinca minor | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Amsonia tabernaemontana | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Catharanthus longifolius | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Ervatamia divaricatum | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Ochrosia elliptica | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Stemmadenia tomentosa var. palmeri | 3-alpha(S)-strictosidine | - |
? | |
tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Voacanga africana | 3-alpha(S)-strictosidine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Catharanthus roseus |
45 | - |
- |
Amsonia salicifolia |
45 | - |
- |
Catharanthus pusillus |
45 | - |
- |
Catharanthus trichophyllus |
45 | - |
- |
Vinca major |
45 | - |
- |
Rauvolfia verticillata |
45 | - |
- |
Vinca minor |
45 | - |
- |
Amsonia tabernaemontana |
45 | - |
- |
Catharanthus longifolius |
45 | - |
- |
Ervatamia divaricatum |
45 | - |
- |
Ochrosia elliptica |
45 | - |
- |
Stemmadenia tomentosa var. palmeri |
50 | - |
- |
Rauvolfia vomitoria |
50 | - |
- |
Amsonia orientalis |
50 | - |
- |
Voacanga africana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Catharanthus roseus |
6 | - |
- |
Amsonia orientalis |
6.8 | - |
- |
Catharanthus roseus |
6.9 | - |
- |
Catharanthus pusillus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | - |
Catharanthus roseus |