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Literature summary for 4.3.2.2 extracted from

  • Lee, P.; Colman, R.F.
    Expression, purification, and characterization of stable, recombinant human adenylosuccinate lyase (2007), Protein Expr. Purif., 51, 227-234.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the pET-14b vector for expression in Escherichia coli Rosetta 2DE3pLysS cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00146
-
adenylosuccinate histidine-tagged enzyme Homo sapiens
0.00178
-
adenylosuccinate none-tagged enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
histidine-tagged enzyme, determined by SDS-PAGE Homo sapiens
222000
-
analytical ultracentrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate Homo sapiens
-
fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?
N6-(1,2-dicarboxyethyl)AMP Homo sapiens
-
fumarate + AMP
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a Ni-NTA column, the 6-histidine tag is removed by cleavage with thrombin, separation of thrombin and histidine-tag from the enzyme is achieved by using a Sephadex G-100 column Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
crude cell lysate Homo sapiens
3.6
-
final purified human enzyme Homo sapiens
3.6
-
imidazole gradient elution Homo sapiens
3.8
-
non tagged enzyme Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, potassium phosphate buffer, KCl, DTT, EDTA, glycerol Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
Homo sapiens fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?
5-aminoimidazole-(N-succinylocarboxamide) ribotide
-
Homo sapiens 5-aminoimidazole-4-carboxamide ribotide + fumarate
-
?
adenylosuccinate
-
Homo sapiens AMP + fumarate
-
?
N6-(1,2-dicarboxyethyl)AMP
-
Homo sapiens fumarate + AMP
-
?

Subunits

Subunits Comment Organism
tetramer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
adenylosuccinate lyase
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
enzyme is stable for at least 5 days at 25°C, and upon rapid freezing and thawing Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
activity assay Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.2 8.4
-
Homo sapiens