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Literature summary for 4.3.2.2 extracted from
- Expression, purification, and kinetic characterization of recombinant human adenylosuccinate lyase (1993), J. Biol. Chem., 268, 19710-19716.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| normal and mutant enzyme S413P, expression in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | - |
Homo sapiens |  |
| fumarate | noncompetitive | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pH-dependence of Km-value | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
4 * 50000, SDS-PAGE | Homo sapiens |
| 196000 | - |
wild-type enzyme, gel filtration | Homo sapiens |
| 201000 | - |
mutant enzyme S413P, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide | Homo sapiens | reaction in the biosynthesis of AMP | 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate | - |
? | |
| N6-(1,2-dicarboxyethyl)AMP | Homo sapiens | reaction in the biosynthesis of AMP | fumarate + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
normal and mutant enzyme S413P | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| normal and mutant enzyme S413P | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C or -20°C, recombinant enzyme is very unstable regardless of salt or glycerol concentration | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide | reaction in the biosynthesis of AMP | Homo sapiens | 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate | - |
? | |
| N6-(1,2-dicarboxyethyl)AMP | reaction in the biosynthesis of AMP | Homo sapiens | fumarate + AMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 50000, SDS-PAGE | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pH-dependence of turnover-numbers | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Homo sapiens |
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