Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.3.2.2 extracted from

  • Casey, P.J.; Lowenstein, J.M.
    Purification of adenylosuccinate lyase from rat skeletal muscle by a novel affinity column. Stabilization of the enzyme, and effects of anions and fluoro analogues of the substrate (1987), Biochem. J., 246, 263-269.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.2.2

General Stability

General Stability Organism
stable to thawing and refreezing at a protein concentration of above 2 mg/ml Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
erythro-beta-fluoro-4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide
-
 Rattus norvegicus
erythro-beta-fluoroadenylosuccinate
-
 Rattus norvegicus
erythro-beta-fluoroaspartate
-
 Rattus norvegicus
I- 100-200 mM Rattus norvegicus
NO3- 100-200 mM Rattus norvegicus
PO43- activates at low concentration, inhibits at high concentrations above 25 mM Rattus norvegicus
SO42- activates at low concentrations, inhibits at concentrations above 25 mM Rattus norvegicus
threo-beta-4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide
-
 Rattus norvegicus
threo-beta-fluoroadenylosuccinate
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide
-
 Rattus norvegicus
0.0015
-
 N6-(1,2-dicarboxyethyl)AMP
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
PO43- activates at low concentration, inhibits at high concentrations above 25 mM Rattus norvegicus
SO42- activates at low concentrations, inhibits at concentrations above 25 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 4 * 52000, SDS-PAGE Rattus norvegicus
200000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide Rattus norvegicus reaction in the biosynthesis of AMP 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
N6-(1,2-dicarboxyethyl)AMP Rattus norvegicus reaction in the biosynthesis of AMP fumarate + AMP
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
muscle skeletal muscle Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, stable for several weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(5-phosphoribosyl)-4-(N-succinocarboxamide)-5-aminoimidazole i.e. 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide ? Rattus norvegicus 5'-phosphoribosyl-5-amino-4-imidazolecarboxamide + fumarate
-
 ?
4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide reaction in the biosynthesis of AMP Rattus norvegicus 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
N6-(1,2-dicarboxyethyl)AMP i.e. adenylosuccinate Rattus norvegicus fumarate + AMP
-
 ?
N6-(1,2-dicarboxyethyl)AMP reaction in the biosynthesis of AMP Rattus norvegicus fumarate + AMP
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 52000, SDS-PAGE Rattus norvegicus