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Literature summary for 4.3.2.1 extracted from

  • Paul, A.; Mishra, A.; Surolia, A.; Vijayan, M.
    Cloning, expression, purification, crystallization and preliminary X-ray studies of argininosuccinate lyase (Rv1659) from Mycobacterium tuberculosis (2013), Acta Crystallogr. Sect. F, 69, 1422-1424.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.2.1

Cloned(Commentary)

Cloned (Comment) Organism
gene argH, expression of Rv1659 as His-tagged protein, with an extra methionine at the N-terminus and an extended sequence LEHHHHHH at the C-terminus, in Escherichia coli strain BL21 (DE3) Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme, microbatch-under-oil method, mixing of 0.002 ml of 9.5 mg/ml protein in 25 mM phosphate buffer, pH 7.4, with 0.002 ml of precipitant solution containing 100 mM Bis-Tris, pH 5.5, 25% w/v polyethylene glycol 3350, X-ray diffraction structure determination and analysis at 2.40 A resolution, molecular replacement method Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50900
-
 4 * 50900, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WPY7 gene Rv1659
-
Mycobacterium tuberculosis H37Rv P9WPY7 gene Rv1659
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and dialysis to homogeneity Mycobacterium tuberculosis

Subunits

Subunits Comment Organism
homotetramer 4 * 50900, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
ArgH
-
 Mycobacterium tuberculosis
Rv1659
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism last enzyme in the arginine biosynthesis pathway Mycobacterium tuberculosis