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Literature summary for 4.3.1.19 extracted from
- Molecular evolution of threonine dehydratase in bacteria (2013), PLoS ONE, 8, e80750.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Staphylococcus aureus |
| expressed in Escherichia coli as a His-tagged fusion protein | Bacillus subtilis |
| expressed in Escherichia coli as a His-tagged fusion protein | Escherichia coli |
| expressed in Escherichia coli as a His-tagged fusion protein | Pseudomonas putida |
| expressed in Escherichia coli as a His-tagged fusion protein | Corynebacterium glutamicum |
| expressed in Escherichia coli as a His-tagged fusion protein | Streptomyces griseus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
- |
Corynebacterium glutamicum |
| 35200 | - |
- |
Escherichia coli |
| 37100 | - |
- |
Staphylococcus aureus |
| 42600 | - |
- |
Streptomyces griseus |
| 46500 | - |
- |
Corynebacterium glutamicum |
| 46600 | - |
- |
Bacillus subtilis |
| 46900 | - |
- |
Staphylococcus aureus |
| 54900 | - |
- |
Pseudomonas putida |
| 56100 | - |
- |
Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Corynebacterium glutamicum | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
| Staphylococcus aureus | - |
- |
- |
| Streptomyces griseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Staphylococcus aureus |
| using Ni-NTA chromatography | Bacillus subtilis |
| using Ni-NTA chromatography | Escherichia coli |
| using Ni-NTA chromatography | Pseudomonas putida |
| using Ni-NTA chromatography | Corynebacterium glutamicum |
| using Ni-NTA chromatography | Streptomyces griseus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1000 | - |
pH 8, 22°C | Streptomyces griseus |
| 1470 | - |
pH 8, 22°C | Staphylococcus aureus |
| 7400 | - |
pH 8, 22°C | Staphylococcus aureus |
| 8500 | - |
pH 8, 22°C | Corynebacterium glutamicum |
| 9900 | - |
pH 8, 22°C | Escherichia coli |
| 23000 | - |
pH 8, 22°C | Bacillus subtilis |
| 89000 | - |
pH 8, 22°C | Corynebacterium glutamicum |
| 106000 | - |
pH 8, 22°C | Pseudomonas putida |
| 171000 | - |
pH 8, 22°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-threonine | - |
Staphylococcus aureus | 2-oxobutanoate + NH3 | - |
? |  |
| L-threonine | - |
Bacillus subtilis | 2-oxobutanoate + NH3 | - |
? | |
| L-threonine | - |
Escherichia coli | 2-oxobutanoate + NH3 | - |
? | |
| L-threonine | - |
Pseudomonas putida | 2-oxobutanoate + NH3 | - |
? | |
| L-threonine | - |
Corynebacterium glutamicum | 2-oxobutanoate + NH3 | - |
? | |
| L-threonine | - |
Streptomyces griseus | 2-oxobutanoate + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BsBTD1 | - |
Bacillus subtilis |
| CgBTD1 | - |
Corynebacterium glutamicum |
| CgCTD | - |
Corynebacterium glutamicum |
| EcBTD2 | - |
Escherichia coli |
| EcCTD | - |
Escherichia coli |
| PpBTD2 | - |
Pseudomonas putida |
| SaBTD1 | - |
Staphylococcus aureus |
| SaCTD | - |
Staphylococcus aureus |
| SgBTD1 | - |
Streptomyces griseus |
| threonine dehydratase | - |
Staphylococcus aureus |
| threonine dehydratase | - |
Bacillus subtilis |
| threonine dehydratase | - |
Escherichia coli |
| threonine dehydratase | - |
Pseudomonas putida |
| threonine dehydratase | - |
Corynebacterium glutamicum |
| threonine dehydratase | - |
Streptomyces griseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Staphylococcus aureus |
| 22 | - |
assay at | Bacillus subtilis |
| 22 | - |
assay at | Escherichia coli |
| 22 | - |
assay at | Pseudomonas putida |
| 22 | - |
assay at | Corynebacterium glutamicum |
| 22 | - |
assay at | Streptomyces griseus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 43 | - |
T50: 43°C | Staphylococcus aureus |
| 43 | - |
T50: 43°C | Escherichia coli |
| 43 | - |
T50: 43°C | Pseudomonas putida |
| 43 | - |
T50: 43°C | Corynebacterium glutamicum |
| 47 | - |
T50: 47°C | Corynebacterium glutamicum |
| 47 | - |
T50: 47°C | Streptomyces griseus |
| 53 | - |
T50: 53°C | Staphylococcus aureus |
| 55 | - |
T50: 55°C | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Staphylococcus aureus |
| 8 | - |
assay at | Bacillus subtilis |
| 8 | - |
assay at | Escherichia coli |
| 8 | - |
assay at | Pseudomonas putida |
| 8 | - |
assay at | Corynebacterium glutamicum |
| 8 | - |
assay at | Streptomyces griseus |
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