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Literature summary for 4.3.1.19 extracted from
- Evidence for two distinct effector-binding sites in threonine deaminase by site-directed mutagenesis, kinetic, and binding experiments (2000), Biochemistry, 39, 15136-15143.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| isoleucine | activation below 0.01 mM, strong inhibition above, 50% inhibition at 0.064 mM | Arabidopsis thaliana |  |
| NH4+ | 2.1fold increase in activity | Arabidopsis thaliana | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y449L | concentration of isoleucine needed to reach 50% inhibition increases by a factor 45, two different effector-binding sites are constituted in part by Y449 and Y543 | Arabidopsis thaliana |
| Y543L | concentration of isoleucine needed to reach 50% inhibition increases by a factor 38, two different effector-binding sites are constituted in part by Y449 and Y543 | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Arabidopsis thaliana | |
| isoleucine | activation below 0.01 mM, strong inhibition above, 50% inhibition at 0.064 mM, inhibition can be reversed by valine | Arabidopsis thaliana | |
| Mg2+ | - |
Arabidopsis thaliana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 3fold increase in activity, half-maximal activation at 3.1 mM | Arabidopsis thaliana | |
| Li+ | 2.8fold increase in activity | Arabidopsis thaliana | |
| Na+ | 1.7fold increase in activity | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59800 | - |
4 * 59800, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-threonine | Arabidopsis thaliana | - |
2-oxobutanoate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant threonine deaminase | Arabidopsis thaliana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 400 | - |
recombinant threonine deaminase | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-threonine | - |
Arabidopsis thaliana | 2-oxobutanoate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 59800, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TD | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 1 mol per monomer | Arabidopsis thaliana | |
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