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Literature summary for 4.3.1.19 extracted from

  • Higa, E.H.; Ramaley, R.F.
    Purification and properties of threonine deaminase from the X-1 isolate of the genus Thermus (1973), J. Bacteriol., 114, 556-562.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.1.19

Inhibitors

Inhibitors Comment Organism Structure
Ile feed-back inhibition Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
14
-
 L-Thr
-
 Thermus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000 115000 gel filtration Thermus sp.

Organism

Organism UniProt Comment Textmining
Thermus sp.
-
 X-1
-
Thermus sp. X-1
-
 X-1
-

Purification (Commentary)

Purification (Comment) Organism
partial Thermus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Thermus sp.

Storage Stability

Storage Stability Organism
-20°C, 0.05 M potassium phosphate, pH 7.2, enzyme concentration 0.06 mg/ml, half-life: 4 weeks Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine
-
 Thermus sp. pyruvate + NH3
-
 ?
L-serine
-
 Thermus sp. X-1 pyruvate + NH3
-
 ?
L-threonine
-
 Thermus sp. 2-oxobutanoate + NH3
-
 ?
L-threonine
-
 Thermus sp. X-1 2-oxobutanoate + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85 90
-
 Thermus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 98 60°C: about 30% of maximal activity, 98°C: about 15% of maximal activity Thermus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 1 h, 15% loss of activity Thermus sp.
90
-
 rapid inactivation Thermus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Thermus sp.

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate required Thermus sp.