Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrobaculum aerophilum |
Protein Variants | Comment | Organism |
---|---|---|
D166N | mutant enzyme shows wild-type activity | Pyrobaculum aerophilum |
D172N | inactive mutant enzyme | Pyrobaculum aerophilum |
K140Q | inactive mutant enzyme | Pyrobaculum aerophilum |
K147Q | activity of mutant enzyme is severely attenuated | Pyrobaculum aerophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29467 | - |
x * 29467, calculated from sequence | Pyrobaculum aerophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA with an abasic site | Pyrobaculum aerophilum | the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates | ? | - |
? | |
DNA with an abasic site | Pyrobaculum aerophilum DSM 7523 | the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum aerophilum | Q8ZVK6 | - |
- |
Pyrobaculum aerophilum DSM 7523 | Q8ZVK6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrobaculum aerophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA with an abasic site | the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates | Pyrobaculum aerophilum | ? | - |
? | |
DNA with an abasic site | the substrate specificity for 8-oxoguanine/guanine sites is higher than that for 8-oxoguanine/cytosine sites. apurinic-lyase activity of this enzyme cleaved only 20% of these apurinic-sites at the same time point | Pyrobaculum aerophilum | ? | - |
? | |
DNA with an abasic site | the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates | Pyrobaculum aerophilum DSM 7523 | ? | - |
? | |
DNA with an abasic site | the substrate specificity for 8-oxoguanine/guanine sites is higher than that for 8-oxoguanine/cytosine sites. apurinic-lyase activity of this enzyme cleaved only 20% of these apurinic-sites at the same time point | Pyrobaculum aerophilum DSM 7523 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29467, calculated from sequence | Pyrobaculum aerophilum |
Synonyms | Comment | Organism |
---|---|---|
Pa-AGOG | bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18) | Pyrobaculum aerophilum |
PAE2237 | locus name | Pyrobaculum aerophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
15 min, no notable effect on catalytic activity | Pyrobaculum aerophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
calculated from sequence | Pyrobaculum aerophilum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates. Bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18) | Pyrobaculum aerophilum |