Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
K129S | inactive mutant enzyme | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58134 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58134 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA with an abasic site | the enzyme has little specificity for the base opposite 8-oxoguanine. The enzyme has both DNA glycosylase and DNA lyase (beta-elimination) activity, and the combined glycosylase/lyase activity occurs at a rate comparable with the glycosylase activity alone | Methanocaldococcus jannaschii | ? | - |
? | |
DNA with an abasic site | the enzyme has little specificity for the base opposite 8-oxoguanine. The enzyme has both DNA glycosylase and DNA lyase (beta-elimination) activity, and the combined glycosylase/lyase activity occurs at a rate comparable with the glycosylase activity alone | Methanocaldococcus jannaschii DSM 2661 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mjOgg | - |
Methanocaldococcus jannaschii |
oxoG DNA glycosylase | - |
Methanocaldococcus jannaschii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 50 | the enzyme is 6fold more active at 50°C than at 37°C, glycosylase/lyase activity is very similarto that of the glycosylase activity alone | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
3 h, activity is decreased by one-third | Methanocaldococcus jannaschii |
93 | - |
the enzyme is thermally stable, denaturing at 93 °C | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Methanocaldococcus jannaschii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 9.5 | pH 7.3: about 50% of maximal activity, pH 9.5: about 45% of maximal ativity, glycosylase/lyase activity is very similar to that of the glycosylase activity alone | Methanocaldococcus jannaschii |