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Literature summary for 4.2.99.18 extracted from
- Chinese hamster apurinic/apyrimidinic endonuclease (chAPE1) expressed in sf9 cells reveals that its endonuclease activity is regulated by phosphorylation (2010), FEBS J., 277, 4732-4740.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Sf9 cell | Cricetulus griseus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000048 | - |
18-mer containing P33-labeled tetrahydrofuran | enzyme dephosphorylated by lambda phosphatase, pH 7.5, 22°C | Cricetulus griseus | |
| 0.000081 | - |
18-mer containing P33-labeled tetrahydrofuran | enzyme phosphorylated by casein kinase II, pH 7.5, 22°C | Cricetulus griseus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35500 | - |
x * 35500, SDS-PAGE | Cricetulus griseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetulus griseus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | higher phosphorylation of APE1 in the expression host is correlated to a lower endonuclease activity. The phosphorylation of a higher activity batch of APE1 by casein kinase II decreases the endonuclease activity, and the dephosphorylation of APE1 by lambda phosphatase increases the endonuclease activity | Cricetulus griseus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Cricetulus griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 18-mer containing P33-labeled tetrahydrofuran | - |
Cricetulus griseus | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35500, SDS-PAGE | Cricetulus griseus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.58 | - |
18-mer containing P33-labeled tetrahydrofuran | enzyme phosphorylated by casein kinase II, pH 7.5, 22°C | Cricetulus griseus | |
| 5.7 | - |
18-mer containing P33-labeled tetrahydrofuran | enzyme dephosphorylated by lambda phosphatase, pH 7.5, 22°C | Cricetulus griseus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7200 | - |
18-mer containing P33-labeled tetrahydrofuran | enzyme phosphorylated by casein kinase II, pH 7.5, 22°C | Cricetulus griseus | |
| 12000 | - |
18-mer containing P33-labeled tetrahydrofuran | enzyme dephosphorylated by lambda phosphatase, pH 7.5, 22°C | Cricetulus griseus |
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Release 2023.1 (January 2023)
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