Literature summary for 4.2.99.18 extracted from

Asano, R.; Ishikawa, H.; Nakane, S.; Nakagawa, N.; Kuramitsu, S.; Masui, R.
An additional C-terminal loop in endonuclease IV, an apurinic/apyrimidinic endonuclease, controls binding affinity to DNA (2011), Acta Crystallogr. Sect. D, 67, 149-155.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Geobacillus kaustophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 1.6 A resolution	Geobacillus kaustophilus

Protein Variants

Protein Variants	Comment	Organism
K267A	compared with wild-type, the affinity for the substrate analogue, 21 bp double-stranded DNA containing an apurinic/apyrimidinic-site analogue, is drastically decreased. The dissociation constant of the mutant is 0.38 microM	Geobacillus kaustophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	EndoIV contains two Fe2+ ions and one Zn2+ ion, crystallography data. Fe2+(1) is coordinated by Glu145, Asp179, His214, Glu259, and Fe2+(2) is coordinated by His69, His110 and Glu145	Geobacillus kaustophilus
Zn2+	EndoIV contains two Fe2+ ions and one Zn2+ ion, crystallography data. The Zn2+ ion is coordinated by His182, Asp227, His229	Geobacillus kaustophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus kaustophilus	Q5KX27	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Geobacillus kaustophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
21 bp double-stranded DNA containing an apurinic/apyrimidinic-site analogue	-	Geobacillus kaustophilus	?	the affinity of EndoIV for the substrate analogue is very high and its dissociation constant is less than 0.01 microM. A C-terminal DNA-recognition loop at residues 265-269 that is only present in the long type enzymes contributes to its high affinity for apurinic/apyrimidinic sites	?

Synonyms

Synonyms	Comment	Organism
EndoIV	-	Geobacillus kaustophilus

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Release 2023.1 (January 2023)