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Literature summary for 4.2.99.18 extracted from
- Endoribonuclease activity of human apurinic/apyrimidinic endonuclease 1 revealed by a real-time fluorometric assay (2010), Anal. Biochem., 398, 69-75.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E96A | the mutant does not exhibit any nuclease activity against 88-nt RNA substrate | Homo sapiens |
| H309N | the mutant does not exhibit any nuclease activity against 88-nt RNA substrate | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| K+ | K+ is inhibitory to the native APE1 at 0.2-10 mM with approximately 5fold inhibition | Homo sapiens |  |
| K+ | K+ is inhibitory to the native APE1 at 0.2-10 mM with approximately 5fold inhibition | Rattus norvegicus | |
| Mg2+ | 10-20 mM Mg2+ is inhibitory to the RNA-cleaving activity of APE1 | Homo sapiens | |
| Mg2+ | 10-20 mM Mg2+ is inhibitory to the RNA-cleaving activity of APE1 | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000642 | - |
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | native enzyme | Rattus norvegicus | |
| 0.000424 | - |
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | recombinant enzyme | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | K+ significantly stimulates recombinant APE1 activity at 20 mM by approximately 1.3fold (compared with the absence of K+) | Homo sapiens | |
| K+ | K+ significantly stimulates recombinant APE1 activity at 20 mM by approximately 1.3fold (compared with the absence of K+) | Rattus norvegicus | |
| Mg2+ | ion concentrations ranging from 0.2 to 2 mM Mg2+ promotes catalysis, APE1 is enhanced approximately 2fold (compared with the absence of Mg2+) in the presence of 2 mM Mg2+ | Homo sapiens | |
| Mg2+ | ion concentrations ranging from 0.2 to 2 mM Mg2+ promotes catalysis, APE1 is enhanced approximately 2fold (compared with the absence of Mg2+) in the presence of 2 mM Mg2+ | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, SP-Sepharose column chromatography | Homo sapiens |
| Ni-NTA column chromatography, SP-Sepharose column chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | the fluorogenic substrate OligoI is based on the sequence immediately surrounding the stem V-loop region (OligoI) and incorporating a fluorescent tag, Cy3, at the 5' end and a fluorescence Black Hole Quencher at the 3' end of the oligonucleotide | Homo sapiens | ? | - |
? | |
| 5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | the fluorogenic substrate OligoI is based on the sequence immediately surrounding the stem V-loop region (OligoI) and incorporating a fluorescent tag, Cy3, at the 5' end and a fluorescence Black Hole Quencher at the 3' end of the oligonucleotide | Rattus norvegicus | ? | - |
? | |
| 5'-Cy3-CAAGGTAGTTATCCTTG-1-Black Hole Quencher1-3' | the fluorogenic substrate DNAOligoI is based on the sequence immediately surrounding the stem Vloop region (OligoI) and incorporating a fluorescent tag, Cy3, at the 5' end and a fluorescence Black Hole Quencher at the 3' end of the oligonucleotide, DNAOligoI has an identical sequence to OligoI except that deoxythymidylate is substituted for 2' hydroxyl uridine | Homo sapiens | ? | - |
? | |
| 5'-Cy3-CAAGGTAGTTATCCTTG-1-Black Hole Quencher1-3' | the fluorogenic substrate DNAOligoI is based on the sequence immediately surrounding the stem Vloop region (OligoI) and incorporating a fluorescent tag, Cy3, at the 5' end and a fluorescence Black Hole Quencher at the 3' end of the oligonucleotide, DNAOligoI has an identical sequence to OligoI except that deoxythymidylate is substituted for 2' hydroxyl uridine | Rattus norvegicus | ? | - |
? | |
| c-myc RNA | APE1 cleaves at the UA, CA, and UG sites of c-myc RNA in vitro | Homo sapiens | ? | - |
? | |
| c-myc RNA | APE1 cleaves at the UA, CA, and UG sites of c-myc RNA in vitro | Rattus norvegicus | ? | - |
? | |
| additional information | APE1 at low concentrations does not have any effect on 5'-Cy3-CrUAGGTAGTTATCCrUAG-Black Hole Quencher1-3' (OligoII), under similar conditions, the recombinant APE1 has no effect on fluorescently labeled or 32P-labeled DNAOligoI | Homo sapiens | ? | - |
? | |
| additional information | APE1 at low concentrations does not have any effect on 5'-Cy3-CrUAGGTAGTTATCCrUAG-Black Hole Quencher1-3' (OligoII), under similar conditions, the recombinant APE1 has no effect on fluorescently labeled or 32P-labeled DNAOligoI | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| APE1 | - |
Homo sapiens |
| APE1 | - |
Rattus norvegicus |
| apurinic-apyrimidinic endonuclease I | - |
Homo sapiens |
| apurinic-apyrimidinic endonuclease I | - |
Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0272 | - |
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | recombinant enzyme | Homo sapiens | |
| 0.168 | - |
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | native enzyme | Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | in addition to the apurinic/apyrimidinic DNA endonuclease activity, APE1 has 3'-5' DNA exonuclease, 3' phosphodiesterase, and RNase H activities | Rattus norvegicus |
| physiological function | in addition to the apurinic/apyrimidinic DNA endonuclease activity, APE1 has 3'-5' DNA exonuclease, 3'-phosphodiesterase, and RNase H activities | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 64.15 | - |
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | recombinant enzyme | Homo sapiens | |
| 2620 | - |
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | native enzyme | Rattus norvegicus |
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Release 2023.1 (January 2023)
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