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Literature summary for 4.2.99.18 extracted from

  • Bogani, F.; Boehmer, P.E.
    The replicative DNA polymerase of herpes simplex virus 1 exhibits apurinic/apyrimidinic and 5-deoxyribose phosphate lyase activities (2008), Proc. Natl. Acad. Sci. USA, 105, 11709-11714.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.18

Cloned(Commentary)

Cloned (Comment) Organism
UL30 is expressed in Spodoptera frugiperda cells Human alphaherpesvirus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for AP cleavage is similar to that for 5'-deoxyribose-5-phosphate removal, it explains the relative difference in apurinic/apyrimidinic cleavage observed with the plasmid and apurinic/apyrimidinic duplex oligonucleotide with the former containing a 100fold higher concentration of apurinic/apyrimidinic sites (100 uracil residues per plasmid molecule) than the oligonucleotides-based substrate. Human alphaherpesvirus 1

Metals/Ions

Metals/Ions Comment Organism Structure
additional information cleavage of abasic DNA by UL30 occurs in the presence of EDTA and is independent of Mg2+, UL30 is 10fold less active Human alphaherpesvirus 1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
AP DNA Human alphaherpesvirus 1 AP sites fragments of DNA
-
 ?
additional information Human alphaherpesvirus 1 catalytic subunit of the HSV-1 DNA polymerase (Pol) (UL30) exhibits apurinic/apyrimidinic (AP) and 5'-deoxyribose phosphate lyase activities which are integral to base excision repair and lead to DNA cleavage on the 3'-side of abasic sites and 5'-deoxyribose-5-phosphate residues that remain after cleavage by 5'-AP endonuclease. DNA lyase activity residues in the Pol domain of UL30. ?
-
 ?
additional information Human alphaherpesvirus 1 enzyme has implications on the role of BER in viral genome maintenance during lytic replication and reactivation from latency ?
-
 ?

Organism

Organism UniProt Comment Textmining
Human alphaherpesvirus 1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Human alphaherpesvirus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AP DNA AP sites Human alphaherpesvirus 1 fragments of DNA
-
 ?
linear 31-mer duplex oligonucleotide (3'-32P-labeled top strand contains an abasic site at position 17) cleavage 3' to the apurinic/apyrimidinic site Human alphaherpesvirus 1 3'-32P-labeled 14-mer oligonucleotide
-
 ?
additional information catalytic subunit of the HSV-1 DNA polymerase (Pol) (UL30) exhibits apurinic/apyrimidinic (AP) and 5'-deoxyribose phosphate lyase activities which are integral to base excision repair and lead to DNA cleavage on the 3'-side of abasic sites and 5'-deoxyribose-5-phosphate residues that remain after cleavage by 5'-AP endonuclease. DNA lyase activity residues in the Pol domain of UL30. Human alphaherpesvirus 1 ?
-
 ?
additional information enzyme has implications on the role of BER in viral genome maintenance during lytic replication and reactivation from latency Human alphaherpesvirus 1 ?
-
 ?

Synonyms

Synonyms Comment Organism
apurinic/apyrimidinic lyase catalytic subunit of the HSV-1 DNA polymerase (Pol) (UL30) (EC 2.7.7.7) exhibits apurinic/apyrimidinic and 5'-deoxyribose phosphate lyase activities Human alphaherpesvirus 1
DNA lyase
-
 Human alphaherpesvirus 1
UL30
-
 Human alphaherpesvirus 1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Human alphaherpesvirus 1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Human alphaherpesvirus 1