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Literature summary for 4.2.99.18 extracted from

  • Ibarra, J.R.; Orozco, A.D.; Rojas, J.A.; Lopez, K.; Setlow, P.; Yasbin, R.E.; Pedraza-Reyes, M.
    Role of the Nfo and ExoA apurinic/apyrimidinic endonucleases in repair of DNA damage during outgrowth of Bacillus subtilis spores (2008), J. Bacteriol., 190, 2031-2038.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.18

Cloned(Commentary)

Cloned (Comment) Organism
chromosomal DNA is isolated from Bacillus subtilis, and competent cells are prepared and transformed with plasmid or chromosomal DNA. nfo mutant strains are constructed Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA Bacillus subtilis The latter lesions are processed by AP endonucleases, which are important components of the base excision repair pathway fragments of DNA
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 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
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 strain 168 (wild-type) and strain PS832 (trp+ revertant of strain 168), prototrophic derivative
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Source Tissue

Source Tissue Comment Organism Textmining
spore
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 Bacillus subtilis
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 cells lacking Nfo and ExoA are around 1.2times more susceptible to H2O2 treatment than cells of the parental strain Bacillus subtilis
additional information
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 compared to wild-type spores, nfo and exoA spores exhibit slower outgrowth, although they exhibit essentially identical rates of germination when germination is judged by determining the decrease in the OD600 of spore cultures shortly after addition of spores to germination medium Bacillus subtilis
additional information
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 germination rate of spores lacking both Nfo and ExoA is similar to that of wild-type spores, but the return to vegetative growth (outgrowth) is significantly slower for the nfo exoA spores Bacillus subtilis
additional information
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 The absence of Nfo and ExoA in uvrA and recA spores resulted in a slow-outgrowth phenotype Bacillus subtilis
additional information
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 the mutation frequency in outgrown nfo exoA spores is 10fold higher than that in outgrown wild-type spores treated with H2O2 Bacillus subtilis
additional information
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 the outgrowth of spores lacking the enzyme is slowed, and the spores have an elevated mutation frequency, suggesting that these enzymes repair DNA lesions induced by oxidative stress during spore germination and outgrowth. Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA The latter lesions are processed by AP endonucleases, which are important components of the base excision repair pathway Bacillus subtilis fragments of DNA
-
 ?

Synonyms

Synonyms Comment Organism
AP endonuclease
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 Bacillus subtilis
apurinic/apyrimidinic endonuclease
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 Bacillus subtilis
ExoA
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 Bacillus subtilis
Nfo
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 Bacillus subtilis