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Literature summary for 4.2.99.18 extracted from

  • Jiang, Y.; Guo, C.; Vasko, M.R.; Kelley, M.R.
    Implications of apurinic/apyrimidinic endonuclease in reactive oxygen signaling response after cisplatin treatment of dorsal root ganglion neurons (2008), Cancer Res., 68, 6425-6434.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.18

Activating Compound

Activating Compound Comment Organism Structure
cisplatin cisplatin-induced (chemotherapeutic agent) increase in Api1 expression after exposition with 25 micromol/l or higher concentrations for 24 hours, Api1 is neuroprotective against cisplatin-induced neurotoxity Rattus norvegicus
additional information after infection with adenoviral constructs (mutant C65A) and treatment with 50 micromol/l cisplatin, Ape1 is overexpressed in sensory neuronal rat cell cultures Rattus norvegicus

Application

Application Comment Organism
drug development cancer therapy Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
neuronal cell cultures are transfected with Api1 small interfering RNA, rat neuronal cell cultures are infected with adenovirus Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C65A redox deficient-DNA repair competent (adenovirus infected) Rattus norvegicus
N226A/R177A DNA repair-deficient-redox competent Ape1 does not protect rat neurons from cisplatin toxicity (adenovirus infected) Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information exposing neuronal cultures to Api1 small interfering RNA significantly reduces the expression of Api1 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA Rattus norvegicus multifunctional enzyme involved in DNA base excision repair of oxidative DNA damage and in redox regulation of a number of transcription factors fragments of DNA
-
 ?
additional information Rattus norvegicus increase in phosphorylation of p53 after a decrease in Ape1 levels in sensory neuronal cultures ?
-
 ?
additional information Rattus norvegicus overexpressing wild-type Ape1 attentuates all the toxic effects of cisplatin in cells containing normal endogenous levels of Ape1 and in cells with reduced Ape1 levels after Ape1siRNA treatment ?
-
 ?
additional information Rattus norvegicus reducing expression of Ape1 in neuronal cultures using small interfering RNA enhances cisplatin-induced cell killing, apoptosis, ROS generation and cisplatin-induced reduction in iCGRP release ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Sprague-Dawley rat
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information ganglioneuroma cell, dorsal root ganglions are collected from the spinal column, incubated with collagenase and mechanically dissociated Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 overexpression of Ape1 reduces cisplatin-induced apoptosis in sensory neuronal cell cultures Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA multifunctional enzyme involved in DNA base excision repair of oxidative DNA damage and in redox regulation of a number of transcription factors Rattus norvegicus fragments of DNA
-
 ?
additional information increase in phosphorylation of p53 after a decrease in Ape1 levels in sensory neuronal cultures Rattus norvegicus ?
-
 ?
additional information overexpressing wild-type Ape1 attentuates all the toxic effects of cisplatin in cells containing normal endogenous levels of Ape1 and in cells with reduced Ape1 levels after Ape1siRNA treatment Rattus norvegicus ?
-
 ?
additional information reducing expression of Ape1 in neuronal cultures using small interfering RNA enhances cisplatin-induced cell killing, apoptosis, ROS generation and cisplatin-induced reduction in iCGRP release Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
APE/Ref-1
-
 Rattus norvegicus
APE1
-
 Rattus norvegicus
apurinic/apyrimidinic endonuclease/redox effector factor-1
-
 Rattus norvegicus