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Literature summary for 4.2.99.18 extracted from

  • Chattopadhyay, R.; Wiederhold, L.; Szczesny, B.; Boldogh, I.; Hazra, T.K.; Izumi, T.; Mitra, S.
    Identification and characterization of mitochondrial abasic (AP)-endonuclease in mammalian cells (2006), Nucleic Acids Res., 34, 2067-2076.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
KCl
-
Homo sapiens
KCl
-
Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
into the pET15b vector for expression in Escherichia coli BL21DE3 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens
Mg2+
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
purified APE activity from beef liver mitochondria, determined by SDS-PAGE and Western blot, verified by mass spectrometry Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P23196
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
dialyzed mitochondrial lysates are loaded on a HiTrap Q and SP-Sepharose columns, fractions with APE activity are loaded onto a HiTrap SP column and are further purified using a HiTrap-Heparin column Bos taurus
recombinant human full-length and Ndelta33 APE1 is purified using a Ni-NTA Superflow and a SP-Sepharose column Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Synonyms

Synonyms Comment Organism
abasic (AP)-endonuclease
-
Homo sapiens
abasic (AP)-endonuclease
-
Bos taurus
Ape
-
Homo sapiens
Ape
-
Bos taurus
APE1
-
Homo sapiens
APE1
-
Bos taurus
hAPE1
-
Homo sapiens
mtAPE mtAPE is derived from APE1 after deletion of 33N-terminal residues Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
AP-endonuclease assay Homo sapiens
37
-
AP-endonuclease assay Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
AP-endonuclease assay Homo sapiens
7.5
-
AP-endonuclease assay Bos taurus
9.2
-
AP-endonuclease assay Homo sapiens
9.2
-
AP-endonuclease assay Bos taurus