Cloned (Comment) | Organism |
---|---|
into the pET15b vector for expression in Escherichia coli BL21DE3 cells | Lactiplantibacillus plantarum |
into the pET15b vector for expression in Escherichia coli BL21DE3 cells | Escherichia coli |
into the pET15b vector for expression in Escherichia coli BL21DE3 cells | Thermoplasma volcanium |
Protein Variants | Comment | Organism |
---|---|---|
W199S | mutant is designed in order to investigate the involvement of the tryptophan residues in AP site recognition | Lactiplantibacillus plantarum |
W199S/W213S | mutant is designed in order to investigate the involvement of the tryptophan residues in AP site recognition | Lactiplantibacillus plantarum |
W200S | mutant is designed in order to investigate the involvement of the tryptophan residues in AP site recognition | Thermoplasma volcanium |
W200S/W214S | mutant is designed in order to investigate the involvement of the tryptophan residues in AP site recognition | Thermoplasma volcanium |
W213S | mutant is designed in order to investigate the involvement of the tryptophan residues in AP site recognition | Lactiplantibacillus plantarum |
W214S | mutant is designed in order to investigate the involvement of the tryptophan residues in AP site recognition | Thermoplasma volcanium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31400 | - |
theoretical molecular weight of the hexahistidine-tagged protein, confirmed by SDS-PAGE analysis | Thermoplasma volcanium |
31800 | - |
theoretical molecular weight of the hexahistidine-tagged protein, confirmed by SDS-PAGE analysis | Lactiplantibacillus plantarum |
33100 | - |
theoretical molecular weight of the hexahistidine-tagged protein, confirmed by SDS-PAGE analysis | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Lactiplantibacillus plantarum | - |
- |
- |
Thermoplasma volcanium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a HisTrap HP column | Lactiplantibacillus plantarum |
using a HisTrap HP column | Escherichia coli |
using a HisTrap HP column | Thermoplasma volcanium |
Synonyms | Comment | Organism |
---|---|---|
AP endonuclease | - |
Lactiplantibacillus plantarum |
AP endonuclease | - |
Escherichia coli |
AP endonuclease | - |
Thermoplasma volcanium |
apurinic/apyrimidinic endonuclease | - |
Lactiplantibacillus plantarum |
apurinic/apyrimidinic endonuclease | - |
Escherichia coli |
apurinic/apyrimidinic endonuclease | - |
Thermoplasma volcanium |
DNA-(apurinic or apyrimidinic site) lyase | - |
Lactiplantibacillus plantarum |
DNA-(apurinic or apyrimidinic site) lyase | - |
Escherichia coli |
DNA-(apurinic or apyrimidinic site) lyase | - |
Thermoplasma volcanium |
ExoA type AP endonuclease | - |
Lactiplantibacillus plantarum |
ExoA type AP endonuclease | - |
Escherichia coli |
ExoA type AP endonuclease | - |
Thermoplasma volcanium |
ExoIII | - |
Escherichia coli |
LplExo | - |
Lactiplantibacillus plantarum |
TvoExo | - |
Thermoplasma volcanium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
protein-DNA binding assay | Lactiplantibacillus plantarum |
50 | - |
protein-DNA binding assay | Escherichia coli |
50 | - |
protein-DNA binding assay | Thermoplasma volcanium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
ExoIII rapidly loses activity with preheating at more than 40°C, and almost completely lose it at 55°C | Escherichia coli |
40 | - |
TvoExo gradually loses AP endonuclease activity at more than 40°C, but it retaines about 20% of activity at 80°C | Thermoplasma volcanium |
45 | - |
LplExo does not cleave AP-DNA after preheating at more than 45°C | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
DNA cleavage reaction | Lactiplantibacillus plantarum |
8 | - |
DNA cleavage reaction | Escherichia coli |
8 | - |
DNA cleavage reaction | Thermoplasma volcanium |
8 | - |
protein-DNA binding assay | Lactiplantibacillus plantarum |
8 | - |
protein-DNA binding assay | Escherichia coli |
8 | - |
protein-DNA binding assay | Thermoplasma volcanium |