Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.99.18 extracted from

  • Kaneda, K.; Ohishi, K.; Sekiguchi, J.; Shida, T.
    Characterization of the AP endonucleases from Thermoplasma volcanium and Lactobacillus plantarum: Contributions of two important tryptophan residues to AP site recognition (2006), Biosci. Biotechnol. Biochem., 70, 2213-2221.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the pET15b vector for expression in Escherichia coli BL21DE3 cells Lactiplantibacillus plantarum
into the pET15b vector for expression in Escherichia coli BL21DE3 cells Escherichia coli
into the pET15b vector for expression in Escherichia coli BL21DE3 cells Thermoplasma volcanium

Protein Variants

Protein Variants Comment Organism
W199S mutant is designed in order to investigate the involvement of the tryptophan residues in AP site recognition Lactiplantibacillus plantarum
W199S/W213S mutant is designed in order to investigate the involvement of the tryptophan residues in AP site recognition Lactiplantibacillus plantarum
W200S mutant is designed in order to investigate the involvement of the tryptophan residues in AP site recognition Thermoplasma volcanium
W200S/W214S mutant is designed in order to investigate the involvement of the tryptophan residues in AP site recognition Thermoplasma volcanium
W213S mutant is designed in order to investigate the involvement of the tryptophan residues in AP site recognition Lactiplantibacillus plantarum
W214S mutant is designed in order to investigate the involvement of the tryptophan residues in AP site recognition Thermoplasma volcanium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31400
-
theoretical molecular weight of the hexahistidine-tagged protein, confirmed by SDS-PAGE analysis Thermoplasma volcanium
31800
-
theoretical molecular weight of the hexahistidine-tagged protein, confirmed by SDS-PAGE analysis Lactiplantibacillus plantarum
33100
-
theoretical molecular weight of the hexahistidine-tagged protein, confirmed by SDS-PAGE analysis Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Lactiplantibacillus plantarum
-
-
-
Thermoplasma volcanium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a HisTrap HP column Lactiplantibacillus plantarum
using a HisTrap HP column Escherichia coli
using a HisTrap HP column Thermoplasma volcanium

Synonyms

Synonyms Comment Organism
AP endonuclease
-
Lactiplantibacillus plantarum
AP endonuclease
-
Escherichia coli
AP endonuclease
-
Thermoplasma volcanium
apurinic/apyrimidinic endonuclease
-
Lactiplantibacillus plantarum
apurinic/apyrimidinic endonuclease
-
Escherichia coli
apurinic/apyrimidinic endonuclease
-
Thermoplasma volcanium
DNA-(apurinic or apyrimidinic site) lyase
-
Lactiplantibacillus plantarum
DNA-(apurinic or apyrimidinic site) lyase
-
Escherichia coli
DNA-(apurinic or apyrimidinic site) lyase
-
Thermoplasma volcanium
ExoA type AP endonuclease
-
Lactiplantibacillus plantarum
ExoA type AP endonuclease
-
Escherichia coli
ExoA type AP endonuclease
-
Thermoplasma volcanium
ExoIII
-
Escherichia coli
LplExo
-
Lactiplantibacillus plantarum
TvoExo
-
Thermoplasma volcanium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
protein-DNA binding assay Lactiplantibacillus plantarum
50
-
protein-DNA binding assay Escherichia coli
50
-
protein-DNA binding assay Thermoplasma volcanium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
ExoIII rapidly loses activity with preheating at more than 40°C, and almost completely lose it at 55°C Escherichia coli
40
-
TvoExo gradually loses AP endonuclease activity at more than 40°C, but it retaines about 20% of activity at 80°C Thermoplasma volcanium
45
-
LplExo does not cleave AP-DNA after preheating at more than 45°C Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
DNA cleavage reaction Lactiplantibacillus plantarum
8
-
DNA cleavage reaction Escherichia coli
8
-
DNA cleavage reaction Thermoplasma volcanium
8
-
protein-DNA binding assay Lactiplantibacillus plantarum
8
-
protein-DNA binding assay Escherichia coli
8
-
protein-DNA binding assay Thermoplasma volcanium