Protein Variants | Comment | Organism |
---|---|---|
D44V | mutant retains glycosylase activity against oxidized pyrimidines, but the apparent rate constant for the lyase activity is significantly lower than the wild-type value | Escherichia coli |
R184A | mutant enzyme maintains lyase activity but exhibits glycosylase specificity different from wild-type enzyme | Escherichia coli |
S39L | mutant does not have significant glycosylase activity for oxidized pyrimidines, alhough it maintains AP lysae activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000036 | - |
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G | pH 7.6, 37°C, wild-type enzyme | Escherichia coli | |
0.0000059 | - |
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G | pH 7.6, 37°C, wild-type enzyme | Escherichia coli | |
0.0000091 | - |
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A | pH 7.6, 37°C, wild-type enzyme | Escherichia coli | |
0.0000097 | - |
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A | pH 7.6, 37°C, wild-type enzyme | Escherichia coli | |
0.000133 | - |
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G | pH 7.6, 37°C, mutant enzyme R184A | Escherichia coli | |
0.00192 | - |
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G | pH 7.6, 37°C, mutant enzyme R184A | Escherichia coli | |
0.004 | - |
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A | pH 7.6, 37°C, mutant enzyme R184A | Escherichia coli | |
0.14 | - |
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A | pH 7.6, 37°C, mutant enzyme R184A | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A | - |
Escherichia coli | ? | - |
? | |
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G | - |
Escherichia coli | ? | - |
? | |
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A | - |
Escherichia coli | ? | - |
? | |
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G | - |
Escherichia coli | ? | - |
? | |
DNA containing apurinic/apyrimidinic sites | endonuclease III plays an important cellular role by removing premutagenic pyrimidine damages produced by reactive oxygen species. EcoNth is a bifunctional enzyme that has DNA glycosylase and apurinic/apyrimidinic lyase activity | Escherichia coli | fragments of DNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EcoNth | a bifunctional enzyme that has DNA glycosylase and apurinic/apyrimidinic lyase activity | Escherichia coli |
endonuclease III | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A | pH 7.6, 37°C, wild-type enzyme | Escherichia coli | |
0.043 | - |
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G | pH 7.6, 37°C, wild-type enzyme | Escherichia coli | |
0.075 | - |
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G | pH 7.6, 37°C, wild-type enzyme | Escherichia coli | |
0.098 | - |
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A | pH 7.6, 37°C, wild-type enzyme | Escherichia coli | |
0.098 | - |
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A | pH 7.6, 37°C, mutant enzyme R184A | Escherichia coli | |
0.17 | - |
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G | pH 7.6, 37°C, mutant enzyme R184A | Escherichia coli | |
1.37 | - |
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A | pH 7.6, 37°C, mutant enzyme R184A | Escherichia coli | |
1.6 | - |
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G | pH 7.6, 37°C, mutant enzyme R184A | Escherichia coli |